Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.5.1.4 (
deaminase
)
5,113
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
A novel enantioselective
amidase
screening system was developed and proved to be efficient and accurate. This screening system employed acyl transfer activity of
amidase
in the presence of hydroxylamine, leading to the formation of hydroxamic acids, followed by spectrophotometric quantification of hydroxamic acid/iron(III) complexes. The enantioselectivities of
amidase
were evaluated by employing (R, S)-2, 2-dimethyl
cyclopropanecarboxamide
(1), (S)-2, 2-dimethyl
cyclopropanecarboxamide
and their mixture as substrates concurrently under the same conditions. To prove the accuracy of the screening system, enantioselectivity of acyl transfer reaction (E (T)) and that of hydrolytic reaction (E (H)) was compared. With this method, we obtained eight microorganism strains with enantioselective
amidase
from 523 isolates, two of which showed R-stereospecific avtivity for (R, S)-1.
...
PMID:A screening system for active and enantioselective amidase based on its acyl transfer activity. 1703 73
