Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.5.1.4 (deaminase)
 5,113 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Pseudomonas fluorescens UK-1 has been incubated in basal mineral medium which was 0.5mM in pterine or pterine-6-carboxylic acid. During incubation the test organism splits the pteridine ring by liberating carbon dioxide from position 2. Glucose added to the medium greatly enhances both the growth of the organism and the carbon dioxide formation. Despite the structural similarities between pterine and pterine-6-carboxylic acid, only the degradation products derived from pterine are fluorescent in UV-light. Among the degradation products lumazine, pyrazine-2-carboxylic acid, and pyrazine-2-carboxamide have been identified. Also the activities of pterine deaminase and a carbon dioxide-liberating enzyme have been determined.
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PMID:Studies on the degradation of pterine and pterine-6-carboxylic acid by Pseudomonas fluorescens UK-1. 81 Oct 29

An N-aminated pyrazine analogue of cytidine, in which the pyrimidine N(3) ring nitrogen and C(4) amino group were replaced by a C-amino and an N-amino function, respectively, was prepared as a potential deaminase-resistant cytidine antimetabolite. The nucleoside 1,2-diamino-4-beta-D-ribofuranosylpyrazin-2-onium chloride (6) was a mild cytostatic agent but was neither a substrate for nor an inhibitor of mouse kidney cytidine deaminase. It ionized with a lower pKa than expected. The anion did not undergo the dimerization usually observed with N-imino heterocyclic ylides but unerwent hydrolysis of the 2-amino group to yield a 1-aminopyrazine-2,3-dione nucleoside.
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PMID:Synthesis of an N-aminopyrazinonium analogue of cytidine. 682 47

The mechanism of action of pyrazinamide (PZA) is not known. One hypothesis is that PZA functions as a prodrug of pyrazinoic acid. Susceptibility to PZA correlates with amidase activity of the Mycobacterium tuberculosis isolate in question. PZA-resistant isolates retain susceptibility in vitro to pyrazinoic acid and n-propyl pyrazinoate. Esters of pyrazinoic acid appear to circumvent the requirement for activation by mycobacterial amidase. The MICs of n-propyl pyrazinoate for M. tuberculosis isolates are lower than those of pyrazinoic acid. Further studies to assess the effects of modifications of the alcohol and pyrazine moieties of pyrazinoate esters on in vitro and in vivo antituberculosis activity are under way. This may lead to a candidate compound with enhanced activity against both PZA-susceptible and PZA-resistant M. tuberculosis isolates suitable for clinical development.
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PMID:Activity of n-propyl pyrazinoate against pyrazinamide-resistant Mycobacterium tuberculosis: investigations into mechanism of action of and mechanism of resistance to pyrazinamide. 757 14

A convenient, sensitive, quantitative assay for the measurement of chemotaxis of populations of D. discoideum vegetative amoebae is presented. A strategy for determining the boundary of the bulk of a population of migrating amoebae was devised and is described. This assay employs a dynamic gradient and is independent of deaminase activity. Measurements of chemoattractant capabilities of various pteridines, folates, and mixtures of folate fragments are reported. 2-Amino 4-quinazolinone, a pterin analog without the pyrazine ring nitrogens, is chemotactic. Lumazine, deaminated pterin, inhibits chemotaxis towards pterin but not towards folic acid. Deaminofolic acid is a chemoattractant as are mixtures of lumazine plus aminobenzoylglutamic acid or deaminopteroic acid plus various amino acids. Separately, the components of these mixtures exhibit no ability to stimulate chemotaxis. These mixtures are of fragments that together comprise most of the folate structure. Our results are in accord with separate receptors for pterin vs. folic acid and with a high stringency for pterin reception but a relative tolerance for folate reception. The possibility of using such mixtures to investigate the requirements of various parts of the folate structure for competent signalling is discussed.
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PMID:Quantitative analysis of the behavior of Dictyostelium discoideum amoebae: stringency of pteridine reception. 1181 Jun 95