Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.5.1.4 (
deaminase
)
5,113
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The RidA protein (PF01042) from
Salmonella enterica
is a
deaminase
that quenches 2-aminoacrylate (2AA) and other reactive metabolites. In the absence of RidA, 2AA accumulates, damages cellular enzymes, and compromises the metabolic network.
In vitro
, RidA homologs from all domains of life deaminate 2AA, and RidA proteins from plants, bacteria, yeast, and humans complement the mutant phenotype of a
ridA
mutant strain of
S. enterica
In the present study, a methanogenic archaeon,
Methanococcus maripaludis
S2, was used to probe alternative mechanisms to restore metabolic balance.
M. maripaludis
MMP0739, which is annotated as an aspartate/
glutamate racemase
, complemented a
ridA
mutant strain and reduced the intracellular 2AA burden. The aspartate/
glutamate racemase
YgeA from
Escherichia coli
or
S. enterica
, when provided in
trans
, similarly restored wild-type growth to a
ridA
mutant. These results uncovered a new mechanism to ameliorate metabolic stress, and they suggest that direct quenching by RidA is not the only strategy to quench 2AA.
IMPORTANCE
2-Aminoacrylate is an endogenously generated reactive metabolite that can damage cellular enzymes if not directly quenched by the conserved
deaminase
RidA. This study used an archaeon to identify a RidA-independent mechanism to prevent metabolic stress caused by 2AA. The data suggest that a gene product annotated as an aspartate/
glutamate racemase
(MMP0739) produces a metabolite that can quench 2AA, expanding our understanding of strategies available to quench reactive metabolites.
...
PMID:Expression of Pyridoxal 5'-Phosphate-Independent Racemases Can Reduce 2-Aminoacrylate Stress in Salmonella enterica. 2944 Feb 54
