Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.5.1.4 (
deaminase
)
5,113
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Previous investigation [Tsui et al. (1996) Biochim. Biophys. Acta 1269: 41-46] showed that two active forms of alcohol dehydrogenase can be purified from grass carp. The use of a protease inhibitor and the results of SDS-PAGE analysis of the enzymes suggest that one form (
ADH
-C) is a proteolytic product of the other (
ADH
-I). In this study, the protease responsible for the cleavage was purified. The cleavage enzyme had a subunit molecular weight of 28 kDa. An inhibitor study identified it as a serine protease. It exhibited a strong chymotrypsin activity in both esterase and
amidase
assays with a pH optimum in the range 7.5-8.5. The purified chymotrypsin also cleaved the intact grass carp
ADH
-I into the two-fragment
ADH
-C, with an accompanying increase in enzyme activity. A similar effect was not found using horse liver alcohol dehydrogenase.
...
PMID:Identification of an "alcohol dehydrogenase-activating" protease in grass carp hepatopancreas as a chymotrypsin. 944 19
