Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.5.1.4 (deaminase)
 5,113 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Two molecular forms of cobalt-activated acylase present in human tissues and one or three in sera of patients with viral hepatitis were noted. They have different substrate specificity. Only form 2 is strongly inhibited by alpha-hydroxyisocaproyl-tyrosine and -phenylalanine. Electrophoretic migrations of all enzyme forms are different from those of aminoacylase. Immunoglobulin antiform 2 of the acylase does not precipitate other forms of cobalt-activated acylase or aminoacylase.
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PMID:Molecular forms of cobalt-activated acylase in human tissues and serum of patients with viral hepatitis. 44 40

alpha-Hydroxyisocaproyltyrosine (HyIc-Tyr-OH), a potent competitive inhibitor of the cobalt-activated acylase form 2, was synthesized. Its derivative, alpha-aminopentyl-HyIc-Tyr-OEt was coupled to cyanogen bromide-activated Sepharose 4B and was used for about 100-fold purification of the acylase from human liver by affinity chromatography. The preparation obtained did not show aminoacylase, aspartyl acylase or alanylarylamidase activities. The same chromatographic method was also applied to isolate form 2 of the serum acylase from patients with viral hepatitis and guinea pig placenta.
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PMID:Purification of cobalt-activated acylase by affinity chromatography. 57 48

Newly synthesized and non-toxic acyl derivatives of p-aminobenzoic acid were used as substrates indiagnostic kit for assay of cobalt-activated acylase activity. The enzyme activity, in serum of patients with viral hepatitis, depends on time, type and treatment of the disease and also on age and sex of patients. The presence of HBs antigen has no influence on it. In the patient sera 1-3 molecular forms of the enzyme were found but in the liver of healthy or sick individuals two forms were noted. Using alpha-hydroxy-isocaproyl-tyrosine covalently coupled to Sepharose 4B as a bioadsorbent; the form 2 of acylase from human liver was isolated and separated from the form 1, aminoacylase and aspartyl acylase. Specific immunoglobulins anti-form 2 does not react with other forms of the enzyme either in serum or in the liver.
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PMID:Cobalt-activated acylase in serum of patients with viral hepatitis. 74 9

Activity of Coiactivated acylase in the serum was investigated in 120 children aged 3 months to 15 years, divided into five age groups, suffering from viral hepatitis (VH). No differences were found in the mean values of acylase activity between the ages of 3 months and 6 years, and progressively rising values in older age groups. Statistical analysis showed significance of the difference between the youngest age group and the group of children aged 12-15 years. A study of the dynamics of acylase activity in the course of the disease in 20 children showed rapidly declining values of this enzyme activity. Presence of HBAg in the serum was demonstrated in 12 children, in whom levels of acylase activity in the serum were similar to those in children without HB antigenemia, but the small number of cases examined does not permit conclusions. Acylase activity increases with age. This enzyme activity was observed in the serum of 96%, and HBAg in 10% of the examined children.
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PMID:Activity of cobalt-activated acylase and Australia antigen (HBAg) in children suffering from viral hepatitis. 96 18

Cobalt-activated acylase (Co-A) and transaminase activity were determined in the serum of A/Jax, DBA/2 and C3H mice several days after an intraperitoneal injection of 1,000 lethal doses of murine hepatitis virus type 3 (MHV3). A significant rise in the enzyme activity was observed 1 day after the injection, followed by a decrease on day 2. In the case of the genetically resistant A/Jax strain, the Co-A level regularly decreased to reach normal values on days 7-8. On the contrary, among the fully susceptible DBA/2 strain mice (all dead on day 5), a second rise in acylase (Co-A) level was observed on days 3 and 4, much higher than the day-1 values. Among the mice of C3H strain, which is recorded as 'semi-susceptible', some individuals behaved like the susceptible DBA/2. The comparison of serum acylase activity with other liver function tests showed a correlation between Co-A and transaminases (ALT and AST) with C3H and DBA/2 strains, but no correlation with A/JAX resistant strain. gamma-Glutamyltransferase was not detectable in the serum of different strains during the time of experimentations. Our results suggest that Co-A activity correlates with the clinical course, and that Co-A is a sensitive indicator enzyme in the early phase of viral hepatitis.
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PMID:A genetic study of serum levels of cobalt-activated acylase among susceptible, resistant and semiresistant strains of mice with experimental viral hepatitis. 715 76