Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.5.1.12 (biotinidase)
 392 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

In order to evaluate the clinical significance of serum biotin and biotinidase in liver disease, serum biotin levels and biotinidase activities were determined in 83 patients with various liver diseases and 10 healthy controls. Serum biotin levels and biotinidase activities were determined by a simplified lactobacillus plantarum bioassay and liquid chromatography with fluorimetric detection respectively. Serum biotin levels in decompensated liver cirrhosis, hepatoma and fulminant hepatitis were found to be significant low compared with healthy controls, while it was significant high in autoimmune hepatitis. There was no significant difference between serum biotin levels in the other liver diseases and healthy controls. In various liver diseases except for both acute hepatitis and alcoholic liver disease biotinidase activities were significantly reduced than in healthy controls. Serum biotinidase activities were correlated with serum albumin, prothrombin time, ChE and total cholesterol respectively, suggesting that biotinidase activities may reflect the degree of liver damage. These results seem that biotin deficiency may occur in some cases of severe liver diseases.
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PMID:[Clinical evaluation of serum biotin levels and biotinidase activities in patients with various liver diseases]. 238 84

A reproducible, high-yield high-performance gel-permeation chromatographic method for proteins was developed and applied to the final step of purification of human serum biotinidase. One diol-type silica gel column, Tosoh TSK-gel 3000 SW (300 mm x 7.9 mm I.D.; average pore size 30 nm), and two Jasco Bio-Fine GFC SI 150-K columns (300 mm x 7.9 mm I.D.; average pore size 15 nm) were connected in series. A 0.1 M sodium phosphate buffer (pH 6.0) solution containing 0.3 M sodium chloride, glycerol (2.5%, v/v) and the non-ionic detergent Nonidet P-40 (NP-40, 0.15%, v/v) was utilized as an eluent. Recovery of the protein bovine serum albumin from the separating columns was measured by a spectrophotometric method and found to be 77.0 +/- 3.74% (mean +/- S.D.). The recovery of the total activity of human serum biotinidase was 72.6 +/- 13.0%. Since biotinidase activity was not recovered from the column in the absence of NP-40, the introduction of this non-ionic detergent to the mobile phase was shown to be essential for the final purification step of human serum biotinidase.
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PMID:High-recovery protein purification by high-performance gel-permeation chromatography: application to human serum biotinidase. 837 15