Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.5.1.1 (asparaginase)
 2,695 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Asparaginase-resistant subline L5178Y/Asp of murine lymphoblastic leukemia L5178Y cells was established from resistant clones isolated from soft agar cultures treated with Escherichia coli asparaginase. The L5178Y/Asp cells were found to be over 1000-fold more resistant to E. coli asparaginase, co-resistant to inhibitory effect of guinea pig serum (complement), more tumorigenic for syngeneic DBA/2 mice, and to grow much better (higher plating efficiency) in soft agar medium. With the use of the resistant subline in immunocytotoxicity-cytostasis test in the presence of guinea pig complement, we have been able to demonstrate the presence of tumor-associated antigen(s) on the L5178Y cells which is/are also demonstrable by enzyme-linked immunosorbent assay.
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PMID:Establishment of asparaginase- and guinea pig complement-resistant subline of murine lymphoblastic leukemia L5178y cells. 639 Feb 88