Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.5.1.1 (
asparaginase
)
2,695
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Isoenzyme II of Escherichia coli
L-asparaginase
(L-asparagine amidohydrolase,
EC 3.5.1.1
) is among the few enzymes of major therapeutic importance, being used in the treatment of acute lymphoblastic leukemia. We have constructed several inducible expression systems that overproduce
asparaginase II
from recombinant plasmids. The most efficient of these systems consists of plasmid pTWE1, a derivative of
pT7
-7, and an ansB- strain of E. coli, CU1783. These cells produce and secrete amounts of
asparaginase II
that account for 10-15% of the total cellular protein. Most of the active recombinant enzyme can be released from the periplasmic space by a simple osmotic shock procedure. From the resulting material homogeneous
asparaginase II
was obtained by a two-step procedure. Overall yields of purified
asparaginase
were 10-15 mg
asparaginase II
per liter of E. coli culture. The recombinant enzyme appeared identical to conventionally purified preparations.
...
PMID:Construction of expression systems for Escherichia coli asparaginase II and two-step purification of the recombinant enzyme from periplasmic extracts. 182 83
