Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.5.1.1 (
asparaginase
)
2,695
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Crude extracts of Thermoactinomyces vulgaris 13 M.E.S. were prepared by different procedures and their
L-asparaginase
activity was compared. The optimum conditions for the enzyme activity in the crude extract was exerted at pH 8.8, using borate or tris-HCl buffer, or at pH 7.4, using phosphate buffer, after a reaction time of 30 minutes at 50-55 degrees C, using 1.35-5.4 mg protein of the crude extract. The enzyme showed an apparent km value of 4.5 x 10(-3), was more thermostable in crude extracts than in whole cells, and was inhibited by
HgCl2
, KCN, DL-asparagine, L-aspartic acid, and ammonium hydroxide. Enzyme purification by alcohol precipitation and gel filtration was attempted.
...
PMID:L-asparaginase activity in cell-free extracts of Thermoactinomyces vulgaris 13 M.E.S. 664 56
