Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.5.1.1 (
asparaginase
)
2,695
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The nutritional requirements and culture conditions affecting biosynthesis of
L-asparaginase
in a mutant of Escherichia coli
HAP
designated strain A-1 were studied. Asparaginase activity was increased by the addition of L-glutamic acid, L-glutamine, or commercial-grade monosodium glutamate. The rate of enzyme synthesis was dependent on the interaction between the pH of the culture and the amount of oxygen dissolved in the medium. A critical oxygen transfer rate essential for
asparaginase
formation was identified, and a fermentation procedure is described in which enzyme synthesis is controlled by aeration rate. Enhancement of
L-asparaginase
activity by monosodium glutamate was inhibited by the presence of glucose, culture pH, chloramphenicol, and oxygen dissolved in the fermentation medium.
...
PMID:Effect of culture conditions on synthesis of L-asparaginase by Escherichia coli A-1. 1 9
