Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.5.1.1 (
asparaginase
)
2,695
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Agricultural wastes such as the peels of onion and garlic were used as a supplement along with l-asparagine for the very first time to produce increased yield of l-
asparaginase
by
Pseudomonas plecoglossicida
RS1
. Statistical optimization strategies such as response surface methodology were used to generate a medium composition containing extracts of 0.9 (v/v) of garlic peel waste and 0.5% (v/v) onion peel waste along with 0.2% (w/w) l-asparagine, which yielded a twofold increase in the enzyme activity compared to the unsupplemented minimal (M-9) medium. The presence of l-asparagine content in the peel extract was confirmed by high-performance liquid chromatography. Further, l-
asparaginase
was purified to homogeneity, and identity was confirmed by matrix-assisted laser desorption ionization time-of-flight analysis. The application of the purified l-
asparaginase
as a therapeutic was studied in HeLa cells which showed a p53-mediated G
2
cell cycle arrest. Moreover, the purified l-
asparaginase
showed effective acrylamide mitigation in vitro, at 6 IU, and its effective degradation was also demonstrated by the effect on chemotactic index of
Caenorhabditis elegans
and the restoration of the cognitive abilities of
C. elegans
which was coexposed to acrylamide and l-
asparaginase
compared to that exposed to acrylamide alone. Thus, l-
asparaginase
, with multipotent applications, was produced by effective waste utilization for economical commercial production.
...
PMID:Agro Waste Utilization for Cost-Effective Production of l-Asparaginase by
Pseudomonas plecoglossicida
RS1 with Anticancer and Acrylamide Mitigation Potential. 3002 74
