Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.5.1.1 (
asparaginase
)
2,695
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The growth curves of engineered strain JM105(pASN) were different in LB and M-3 media. The expression level and activity of
L-asparaginase
were affected apparently by both biomass and induction time. Glucose repression of production of
L-asparaginase
was found. The stability of the recombinant plasmid pASN in different host strains and in LB and M-3 media was determined. After cultivation inLB broth and M-3 media at 30 degrees C for more than 50 generations without antibiotic selection, then induced at 42 degrees C for up to 5 h, the engineered strains were proved to be stable, except for
DHA
alpha (pASN).
...
PMID:[Culture conditions of engineered strain of L-asparaginase and the recombinant plasmid stability]. 1255 61
