Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.5.1.1 (
asparaginase
)
2,695
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
In T. thermophila two forms of
L-asparaginase
(
EC 3.5.1.1
) were extracted and purified to near homogeneity which are associated with membranes. These two forms of
L-asparaginase
, I or II, act optimally at pH 8.6 and do not present any glutaminase or kinase activity. Both activities reach maximal values at the stationary phase of growth of T. thermophila. L-Asparaginases are solubilized by treatment of the particulates with 2% w/v Triton X-100 and then by sodium phosphate buffer pH 8.0. Both forms cross reacted with antibodies raised against T. pyriformis
L-asparaginase
and show isoelectric points 7.4 and 8.2. Among the metals tested, Ca2+ is the most effective in activating
L-asparaginase
I or II activity.
Sorbitol
alone up to 30% w/v in the assay mixture activates more than 10 x fold the activity of
L-asparaginase
II. Incubation of
L-asparaginase
I or II with increasing concentration of phospholipase C results in gradually loss of their activities. The relative effectiveness of a variety of phospholipids to reconstitute enzyme activity is presented as well.
...
PMID:Two forms of L-asparaginase in Tetrahymena thermophila. 801 91
