Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.4.25.1 (proteasome)
 28,817 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The aim of this study was to investigate the effects of naturally occurring protocatechuic, chlorogenic and tannic acids on the skin tumor promoter, 12-O-tetradecanoylphorbol-13-acetate (TPA), induced NF-kappaB in mouse epidermis. The topical application of these phenolics 15min prior to TPA resulted in a significant decrease in the NF-kappaB activation which was measured in terms of p65-DNA binding. Tannic acid was the most potent inhibitor of the TPA-stimulated p65-DNA binding, while chlorogenic acid was the least effective compound. Tannic acid also reduced the most the NF-kappaB p65 subunit translocation from cytosol to the nucleus and enhanced the retention of IkappaBalpha in the cytosol. Although protocatechuic acid decreased p65-DNA binding, it did not affect TPA-stimulated degradation of IkappaBalpha. All the tested compounds inhibited the IkappaBalpha kinase (IKK) activity in mouse epidermis. Tannic acid was the most potent inhibitor and protocatechuic acid the weakest. Tannic and chlorogenic acids reduced the TPA-induced C-L activity of proteasome 20S to a similar extent. The blockade of upstream kinase IKK signaling by tannic acid, but also by protocatechuic acid, inhibited the enzyme level and the activity of COX-2. Protocatechuic acid also diminished the level and activity of TPA-induced iNOS, which might be related to its weak effect on IkappaBalpha degradation. Our earlier studies demonstrated that these compounds, particularly tannic acid, reduced the formation of the polycyclic aromatic hydrocarbon-DNA adducts in vitro and in vivo in mouse epidermis. The results of our present study indicate that the compounds which reduce the formation of electrophilic PAH metabolites may also diminish NF-kappaB activation. Thus, the phenolic acids, particularly tannic acid, by affecting the key events of initiation and promotion stage of carcinogenesis, have become of great interest for the prevention of cancer.
 ...
PMID:Naturally occurring phenolic acids inhibit 12-O-tetradecanoylphorbol-13-acetate induced NF-kappaB, iNOS and COX-2 activation in mouse epidermis. 2002 73

A delicate intracellular balance among protein synthesis, folding, and degradation is essential to maintaining protein homeostasis or proteostasis, and it is challenged by genetic and environmental factors. Molecular chaperones and the ubiquitin proteasome system (UPS) play a vital role in proteostasis for normal cellular function. As part of protein quality control, molecular chaperones recognize misfolded proteins and assist in their refolding. Proteins that are beyond repair or refolding undergo degradation, which is largely mediated by the UPS. The importance of protein quality control is becoming ever clearer, but it can also be a disease-causing mechanism. Diseases such as phenylketonuria (PKU) and hereditary tyrosinemia-I (HT1) are caused due to mutations in PAH and FAH gene, resulting in reduced protein stability, misfolding, accelerated degradation, and deficiency in functional proteins. Misfolded or partially unfolded proteins do not necessarily lose their functional activity completely. Thus, partially functional proteins can be rescued from degradation by molecular chaperones and deubiquitinating enzymes (DUBs). Deubiquitination is an important mechanism of the UPS that can reverse the degradation of a substrate protein by covalently removing its attached ubiquitin molecule. In this review, we discuss the importance of molecular chaperones and DUBs in reducing the severity of PKU and HT1 by stabilizing and rescuing mutant proteins.
 ...
PMID:Protein Degradation and the Pathologic Basis of Phenylketonuria and Hereditary Tyrosinemia. 3267 6