Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.24.B1 (
angiotensin-converting enzyme 2
)
1,025
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
We have expressed a series of truncated spike (S) glycoproteins of SARS-CoV and found that the N-terminus 14-502 residuals were sufficient to bind to SARS-CoV susceptible Vero E6 cells. With this soluble S protein fragment as an affinity ligand, we screened HeLa cells transduced with retroviral cDNA library from Vero E6 cells and obtained a HeLa cell clone which could bind with the S protein. This cell clone was susceptible to HIV/SARS pseudovirus infection and the presence of a functional receptor for S protein in this cell clone was confirmed by the cell-cell fusion assay. Further studies showed the susceptibility of this cell was due to the expression of endogenous
angiotensin-converting enzyme 2
(
ACE2
) which was activated by inserted
LTR
from retroviral vector used for expression cloning. When human
ACE2
cDNA was transduced into NIH3T3 cells, the
ACE2
expressing NIH3T3 cells could be infected with HIV/SARS pseudovirus. These data clearly demonstrated that
ACE2
was the functional receptor for SARS-CoV.
...
PMID:Expression cloning of functional receptor used by SARS coronavirus. 1476 27
