Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.24.69 (
botulinum neurotoxin
)
1,901
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Neurotransmitter transporters are regulated through a variety of signal transduction mechanisms which appear to operate in order to maintain appropriate levels of transmitter in the synaptic cleft. One such mechanism is the trafficking of the transporter in association with synaptic vesicle release machinery. This report examines the specifics of trafficking regulation of the rat brain GABA transporter
GAT1
by syntaxin 1A, a plasma membrane component of the SNARE complex which is involved in vesicle membrane fusion. In hippocampal neurons,
botulinum neurotoxin
1C, which specifically cleaves syntaxin 1A, down-regulates plasma membrane
GAT1
levels as assessed by surface biotinylation, suggesting that syntaxin 1A is a positive regulator of
GAT1
surface expression. The up-regulation correlates with a decrease in intracellular
GAT1
levels and is complete within several minutes. These data suggest that syntaxin 1A mediates the redistribution of
GAT1
on a time scale important for the rapid regulation of extracellular GABA levels. Expression of different syntaxin 1A constructs in Xenopus oocytes suggests that several portions of the syntaxin 1A molecule are required for the trafficking of
GAT1
. These data suggest that the trafficking of
GAT1
will be subject to regulatory control by the many molecules known to interact with various domains of syntaxin 1A.
...
PMID:Syntaxin 1A up-regulates GABA transporter expression by subcellular redistribution. 1139 10
