Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.24.64 (
MPP
)
1,876
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
This study aims to investigate the function and molecular mechanisms of
Tribbles homolog 3
(
TRB3
) on the
MPP
+
/MPTP-induced Parkinson's disease (PD). In this study,
MPP
+
-induced PD cellular model and MPTP-caused PD mice model were established. Following the transfection with
TRB3
-shRNA, cell viability, cell apoptosis, ROS level, and the ratio of p-p38/ p38, p-JNK/JNK, p-AKT/AKT were examined. At the same time, behavior assessment of wild type female C57BL/6 mice and whole-body
TRB3
knockout mice PD models caused by MPTP were performed by Rotarod test and Open-field test. The results showed that
TRB3
was markedly upregulated in
MPP
+
-induced cellular model through ATF4/CHOP pathway. Knockdown of
TRB3
significantly decreased the
MPP
+
-induced reduction of cell viability, augment of cell apoptosis and accumulation of ROS, inhibited the phosphorylation of p38 and JNK, and promoted the phosphorylation of AKT, in vitro. Further, knockout of
TRB3
improved the behavior impairment of PD mice induced by MPTP, in vivo. In conclusion, knockdown of
TRB3
has a neuroprotective effect on MPTP/
MPP
+
-induced PD cellular and mice models, through regulating MAPK and AKT signaling pathways.
...
PMID:Knockdown of TRB3 improved the MPP
+
/MPTP-induced Parkinson's disease through the MAPK and AKT signaling pathways. 3128 65
