Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.24.64 (
MPP
)
1,876
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Background
: Parkinson's disease (PD) is a
degenerative disease
of central nervous system. 1-Methyl-4-phenylpyridine (
MPP
+
) is a non-selective dopaminergic neurotoxin that induces cell injury similar to PD. This study aimed to explore the protective effects of
Radix Ophiopogonis
polysaccharide extracts (ROP) on the
MPP
+
-induced PC-12 cell injury.
Methods
: PC-12 cells were exposed to
MPP
+
with or without ROP treatment. Then the cell viability, apoptosis, reactive oxygen species (ROS) level, calcium (Ca
2+
) concentration, mitochondrial membrane potential (MMP), Cyctochrome C release, mitochondrial ATP synthesis, and the expression level of Notch signaling pathway were detected by CellTiter 96 AQ
ueous
One Solution Cell Proliferation assay, fluorescent staining, flow cytometer analysis, and western blotting, respectively.
Results
:
MPP
+
treatment obviously induced PC-12 cell injury as evidenced by the cell viability loss and cell apoptosis enhancement.
MPP
+
markedly increased the concentrations of ROS and Ca
2+
and the mitochondrial dysfunction in PC-12 cells. Moreover, the activation of Notch signaling pathway was found after
MPP
+
treatment. ROP significantly reversed the
MPP
+
-induced PC-12 cell viability loss, apoptosis increase, intracellular oxidative stress and endoplasmic reticulum (ER) stress rise, mitochondrial dysfunction and the activation of Notch signaling pathways in PC-12 cells.
Conclusion
: ROP protected PC-12 cells from the
MPP
+
-induced injury through suppressing the increase of the intracellular oxidative stress and ER stress and activation of Notch signaling pathway. These findings will be helpful for understanding the protective roles of ROP in nerve cell injury and provide potential therapeutic drug for PD.
...
PMID:
Radix Ophiopogonis
polysaccharide extracts alleviate MPP
+
-induced PC-12 cell injury through inhibition of Notch signaling pathway. 3193 91
