Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.24.59 (
MIP
)
4,906
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Dextran sulfate sodium
(
DSS
) induces an inflammatory bowel disease-like colitis in animals. To determine the contribution of epithelium to inflammation in the intestine, we examined the effects of
DSS
in transgenic mice that specifically secrete macrophage inflammatory protein-2 (MIP-2) from the intestinal epithelium. We first confirmed the production of
MIP
-2 from intestinal epithelial cells by Western blots in transgenic mice.
MIP
-2 transgenic mice were therefore an appropriate model to examine the role of epithelial cell chemokines in an inflammatory state induced by
DSS
. We then examined the neutrophil migration into the intestine and the effect of
DSS
on this migration by myeloperoxidase staining. There was an increase of myeloperoxidase-positive neutrophils in the intestine from wild-type and transgenic mice after the
DSS
treatment. Furthermore, the increase of neutrophils under stimulation with
DSS
was confirmed quantitatively by measuring specific tissue myeloperoxidase activities. It was significantly greater in
DSS
-treated
MIP
-2 transgenic mice than in wild-type mice in both the small intestine and colon. These results suggest that the inflammatory effects of
DSS
on both small intestine and colon are enhanced by
MIP
-2 secreted by epithelial cells in the transgenic mice. In conclusion, intestinal epithelial cells can act in concert with other inflammatory stimuli in maintaining inflammation.
...
PMID:Dextran sulfate sodium-induced inflammation is enhanced by intestinal epithelial cell chemokine expression in mice. 1250 94
