Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.4.24.59 (MIP)
 4,906 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

We report here the physical mapping of recurrent chromosome 12q13-q15 breakpoints in cell lines derived from primary myxoid liposarcoma, lipoma, uterine leiomyoma, and pleomorphic adenoma of the salivary glands. In fluorescence in situ hybridization (FISH) experiments, we first mapped the position of the chromosome 12 translocation breakpoint in uterine leiomyoma cell line LM-30.1/SV40 relative to loci COL2A1, D12S4, D12S17, D12S6, D12S6, D12S19, D12S8, and D12S7. It mapped between linkage probes CRI-C86 (D12S19) and p7G11 (D12S8). We then isolated YAC clones using CRI-C86- and p7G11-derived sequence-tagged sites, constructed corresponding YAC contigs of 310 and 800 kb, respectively, and established long-range physical maps of these. Cosmid clones LLNL12NCO1-98C10 and LLNL12NCO1-113D12 were isolated using STSs within the CRI-C86- and the p7G11-derived YAC contigs, respectively, and a mixture of them was used to routinely study the various tumor cell lines by FISH analysis. The chromosome 12 breakpoints of all tumor cell lines tested mapped between cosmids LLNL12NCO1-98C10 and LLNL12NCO1-113D12. None of the breakpoints appeared to map within any of the isolated YAC clones. Furthermore, FISH analysis using cosmid LLNL12-NCO1-144G3, which maps at the CHOP locus, revealed that the chromosome 12 breakpoints in all cell lines of the three benign solid tumors that were tested were located distal to the chromosome 12 translocation breakpoint with the CHOP gene in myxoid liposarcoma cells with t(12;16). In conclusion, our studies seem to indicate that the chromosome 12 breakpoints of myxoid liposarcoma, lipoma, uterine leiomyoma, and pleomorphic adenoma of the salivary glands are all clustered within the 7-cM interval between D12S19 and D12S8, with those of the benign solid tumors distal to CHOP. Finally, the MYF5 gene mapped telomeric to LLNL12NCO1-113D12, and the MIP gene mapped centromeric to the chromosome 12 translocation breakpoint in myxoid liposarcoma cells.
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PMID:Physical mapping of chromosome 12q breakpoints in lipoma, pleomorphic salivary gland adenoma, uterine leiomyoma, and myxoid liposarcoma. 802 Sep 67

Tissues samples of leiomyoma and myometrium obtained intraoperatively were analyzed. For evaluation of the synthesis of MIP-lalpha, MIP-1beta, RANTES, eotaxin, eotaxin-2, interleukin-8, CCR1, CCR3, CCR5, CXCR1, and CXCR2, mRNA isolated from tissues samples of leiomyoma and myometrium was subjected to reverse transcription-PCR and assayed by a semiquantitative method (relative to beta-actin). The content of eotaxin, MIP-1alpha, MIP-1beta, and CCR5 mRNA in leiomyoma tissue was lower than in the myometrium. The concentration of MIP-1beta, CCR5, and eotaxin mRNA in common leiomyoma was much lower than in the myometrium. Eotaxin mRNA expression in myometrial tissue of patients with single nodes was much higher than in those with multiple nodes. Moreover, expression of eotaxin mRNA in common leiomyoma was higher than in proliferating leiomyoma. The concentration of mRNA for interleukin-8 in leiomyoma tissue, as well as the content of mRNA for MIP-1alpha and CCR3 in myometrial tissue increased in patients with submucosal nodes (as distinct from nodes of another location). A direct correlation was revealed between the size of the uterus and concentration of mRNA for interleukin-8 and MIP-1beta in myometrial tissue. The concentration of mRNA for MIP-1alpha and MIP-1beta in leiomyoma tissue negatively correlated with the size of the uterus (maximum size of the node) and duration of leiomyoma, respectively. Our results indicate that chemokines play an important role in the pathogenesis of uterine leiomyoma.
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PMID:Expression of mRNA for chemokines and chemokine receptors in tissues of the myometrium and uterine leiomyoma. 1902 11