Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.24.35 (
matrix metalloproteinase 9
)
2,207
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Tumor spreading is associated with the degradation of extracellular matrix proteins, mediated by the overexpression of
matrix metalloproteinase 9
(
MMP-9
). Although, such overexpression was linked to epigenetic promoter methylation, the role of intragenic methylation was not clarified yet. Melanoma was used as tumor model to investigate the relationship between the DNA intragenic methylation ofMMP9 gene and
MMP-9
overexpression at transcriptional and protein levels. Computational analysis revealed DNA hypermethylation within the intragenic CpG-2 region of MMP9 gene in melanoma samples with high
MMP-9
transcript levels. In vitro validation showed that CpG-2 hotspot region was hypermethylated in the A375 melanoma cell line with highest mRNA and protein levels of
MMP-9
, while low methylation levels were observed in the MEWO cell line where
MMP-9
was undetectable. Concordant results were demonstrated in both A2058 and
M14
cell lines. This correlation may give further insights on the role of
MMP-9
upregulation in melanoma.
...
PMID:MMP-9 overexpression is associated with intragenic hypermethylation of MMP9 gene in melanoma. 2711 78
