Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.4.24.3 (collagenase)
 18,340 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Aryl hydrocarbon hydroxylase (AHH) activity mediated by cytochrome P-450 is present in pig hepatic microsomes [10 nmol.3 mg protein-1.hr-1]. AHH activity was detectable in both hepatocytes and Kupffer cells isolated from pig liver biopsy material. These cells were isolated from needle or wedge biopsy material by collagenase perfusion and incubation with collagenase at 37 degrees. The two cell types were separated from the resulting cell suspension as previously described for whole liver. Kupffer cells were enriched by adherence and were cultured for 24 hr prior to harvesting. Cells were harvested, and cell viability was determined. AHH activity was assayed in Kupffer cell and hepatocyte homogenates. Kupffer cell AHH activity was approximately one-eighth the level detected in hepatocytes. To determine whether this enzyme was present in other macrophages, monocytes were isolated from 10 ml of heparinized peripheral blood using Ficoll-Hypaque and were enriched by adherence. After 24 hr in culture, cell viability was assessed and monocytes were identified by by cytochemical staining. AHH activity was detectable in pig monocyte homogenates, and the AHH level was similar to that in pig Kupffer cells. AHH was also easily detectable in human monocytes. This macrophage AHH activity was compared with AHH activity in rat monocytes, mouse Kupffer cells and mouse peritoneal macrophages. Monocyte AHH was relatively stable in cell culture but decreased rapidly upon storage at -70 degrees. Macrophage AHH activity was depressed following phagocytic activation in vitro by latex beads with a concomitant increase in heme oxygenase activity.
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PMID:Drug-metabolizing enzymes in rat, mouse, pig and human macrophages and the effect of phagocytic activation. 368 28

Intestinal mucosal cells from the rat have been isolated by a new technique involving intravascular perfusion of an intestinal segment with collagenase. Detached cells were flushed from the intestinal lumen with a second perfusion circuit containing an oxygenated buffered solution with 1% bovine serum. Sequential collection of cells at intervals during the period of perfusion revealed that villus-tip cells are recovered first (after 15 min of collagenase perfusion), followed by midvillus (after 25 min) and lower villus cells (after 35 min). The isolated cells were judged intact and viable by the criteria of trypan blue dye exclusion, ultrastructural appearance, and metabolic activity. They were characterized as villus-tip, midvillus, and lower villus-crypt cells by their alkaline phosphatase and sucrase activity, glycoprotein formation, and [3H]thymidine incorporation. Microsomal monooxygenase activity was four to five times greater in villus-tip than in lower villus cells, whereas heme oxygenase exhibited a reverse gradient. The isolated cells synthesized heme and bilirubin under cell culture conditions.
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PMID:Characterization of isolated epithelial cells from rat small intestine. 706 42