Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.4.24.3 (collagenase)
 18,340 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

1. An activator catalysing specifically conversion of latent forms of human leucocyte collagenase and gelatin-specific protease into the active forms, has been isolated from rheumatoid synovial fluid and purified 55-fold with a yield of 16%. 2. Molecular weight of the activator is about 35 000. 3. The activator is thermolabile, and is irreversibly inactivated at pH below 5.5 or in the presence of low concentrations of trypsin or papain; it is resistant to the action of lysozyme, hyaluronidase, diisopropylfluorophosphate, soybean trypsin inhibitor, p-chloromercuribenzoate, iodoacetamide and dithiothreitol. 4. The activator did not show any activity towards collagen, gelatin, casein, haemoglobin, histones, elastin or p-phenylazobenzyloxycarbonyl-peptide.
Acta Biochim Pol 1975
PMID:Isolation, purification and properties of a factor from rheumatoid synovial fluid activating the latent forms of collagenolytic enzymes. 17 Jul 64

In the course of healing of experimental ulcer one could observe a distinct difference between a cornea, subjected to the action of glycerol and a control one. The damage to the chemical structure of collagenous fibers was much smaller than in the control. On the basis of the investigations performed one may conclude that glycerol, through dehydration of the corneal tissue and stimulation of mucopolysaccharide synthesis, suppresses the activity of collagenase. In polarized light this process was manifested by the double refraction phenomenon and in clinical observation--by an accelerated healing of ulcer.
Pol Med Sci Hist Bull
PMID:Effect of dehydration of the cornea with experimentally induced ulcer on collagenase activity. 18 91

Cells from aortas of newborn and adult rabbits were liberated by digestion with trypsin and collagenase. More than 90% of them were viable at the end isolation. Isolated cells were used for electron microscopic examination. In the material obtained from aortas of newborn rabbits endothelial cells, fibroblasts and smooth muscle cells were present. In material from adult rabbits two kinds of endothelial cells, fibroblasts, several varieties of myocytes, fat-laden macrophages and typical foam cells were found. Various forms of myocytes seem to represent different functional stages or specialized kinds of these cells. The presence of fat laden macrophages and foam cells in the aortas of healthy adult rabbits may support the view that these cellular changes so far considered as typical of atherosclerosis are common and may be treated as an exponent of natural process of aging of vascular wall.
Pol Med Sci Hist Bull
PMID:Electron microscopic studies on cells isolated from aorta of newborn and adult rabbits. 122 11

The effect of endotracheal administration of bleomycin (10 mg x kg-1) on hydroxyproline content, and on collagenase and elastase activities in rat lungs was investigated. A significant increase in lung tissue hydroxyproline content on 7th day after bleomycin administration was observed. Similar rise in this amino acid level in bronchoalveolar lavage was observed, however, on 14th day after the drug administration. Collagenase activity was lowered by 17% and 36%, respectively. No changes in elastase activity were found. These data suggest that bleomycin cause the lung fibrosis by restraining the collagenase activity.
Arch Vet Pol 1992
PMID:The influence of endotracheally administered bleomycin on hydroxyproline content and collagenase and elastase activity in rat lungs. 128 2

Proteoglycans (PG) from normal and atherosclerotic rabbits aortas were extracted with 4 M guanidine hydrochloride and digested with collagenase in the presence of protease inhibitors. The contents of uronic acid and hexosamine from PG fractions purified by isopycnic CsCl gradient ultracentrifugation under associative and dissociative conditions were significantly higher in the atherosclerotic aortas (up to 40%) than in the control tissue. The uronic acid/protein ratio increased from 0.7 to 1.3 in the monomers PG fraction of atherosclerotic aortas. Chromatographic separation and electrophoretic analysis of PG monomers indicated the presence of three different subfractions PGI, PGII and PGIII in both groups of animals. The uronic acid/protein ratio in PGI from experimental aorta was increased whereas this ratio in PGIII was decreased compared to contrast tissue. The observed increase of sugar component in the core proteins suggests their over glycosylation.
Acta Biochim Pol 1991
PMID:Composition of proteoglycans from rabbit aorta in the experimentally induced atherosclerosis. 166 61

Latent collagenase has been isolated in pure form from the rheumatoid synovial fluid. The final preparation, activated by trypsin, yielded a collagenase of specific activity 2,227 units/mg. Electrophoresis in sodium dodecyl sulfate polyacrylamide gels revealed a protein doublet of 54 and 50 kDa. Trypsin or HgCl2 activation resulted in disappearance of the doublet and emergence of a new doublet of 47 and 43 kDa. The latent collagenase could also be activated by leucocyte cathepsin G or plasmin. Neither the latent nor the active collagenase from synovial fluid showed any cross-reactivity with the antibodies against leucocyte collagenase. The trypsin activated collagenase degraded collagen type I, II, III giving typical cleavage products but did not degrade type IV and V collagen.
Acta Biochim Pol 1990
PMID:Some properties of latent collagenase from human synovial fluid. 196 84

The adenoidal mast cells were obtained from the adenoids by enzymatic dispersion of the tissue by enzyme collagenase. Mast cells constituted 1-2% of total cell population and were morphologically intact, as judged by light microscopy. They could be sensitized passively in vitro with homologous reaginic serum and released histamine after challenge with specific antigen. The adenoidal mast cells released histamine after challenge with anti-IgE. These cells were unresponsive to the action of compound 48/80 and had low sensitivity to the action of polymyxin B, concanavalin A and ionophore A23187. Our studies have shown that mast cells obtained from adenoids by enzymatic dispersion can be used for experiments in vitro.
Otolaryngol Pol 1989
PMID:[Mast cells of the pharyngeal tonsils]. 247 43

Protease-deficient mutants of Pseudomonas aeruginosa obtained by ethyl methane sulphonate treatment were found to show pleiotropic effect on various extracellular toxin activities. Of eight mutants five were completely deficient in lecithinase and four in collagenase activity. The decreased hemolytic activity was obtained in five mutants and enterotoxin activity in seven mutants as compared to parent strain.
Acta Microbiol Pol 1989
PMID:Pleiotropic changes in the activities of extracellular toxins of Pseudomonas aeruginosa protease-deficient mutants. 248 46

Confluent cultures of Balb c/3T3, L-929 and PZ-32 cells were incubated at 37 degrees C for 16 h in the serum-free culture medium supplemented with ascorbic acid, beta-aminopropionitrile and (5(-3) H)proline. An addition of platelet homogenate to the culture medium increased significantly proline incorporation into proteins and synthesis of the hydroxyproline-containing, collagenase-sensitive proteins.
Acta Biochim Pol 1980
PMID:Stimulation of collagen biosynthesis by platelet homogenate in various cell cultures. 726 79

The influence of 12, 24 and 48 hrs of normobaric hyperoxia on hydroxyproline content and both collagenase and elastase activities in rat lung was investigated. The rise in hydroxyproline level in lung after only 24 hrs of hyperoxia was observed. In bronchoalveolar lavage, however, the level of this amino acid remains unchanged during the whole time of the experiment. Collagenase activity decreased significantly after 12 hrs of hyperoxia, and then decreased gradually. Elastase activity was diminished after 48 hrs of hyperoxia. It is suggested that breathing with pure oxygen induce changes in collagenase activity long before the changes in elastase activity or hydroxyproline content.
Arch Vet Pol 1993
PMID:The influence of normobaric hyperoxia on hydroxyproline content and collagenase and elastase activities in rat lung. 805 48


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