Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.4.24.3 (collagenase)
 18,340 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Binding sites of apolipoprotein (apo) C III on non-parenchymal cells (NPC) isolated from rat liver were found. Apo C III was purified from delipided human plasma very low density lipoproteins. 125I-labeled-apo C III was prepared by chloramine-T method. Non-parenchymal cells were isolated from rat liver by collagenase method. Freshly isolated rat liver NPC bound 125I-labeled apo C III in a saturable way with Kd 0.1-0.55 mumol/L and Bmax 18.1-42.0 ng/ml cell protein. There were about 1.0-4.5 x 10(5) specific binding sites of apo C III on each NPC. Unlabeled apo C III, but not apo AI and B100, could inhibit these binding activities. The results demonstrate the presence of saturable and specific apo C III receptors (binding sites) on rat liver NPC, but their nature and biologic properties remain to be investigated.
Hua Xi Yi Ke Da Xue Xue Bao 1992 Sep
PMID:[Apolipoprotein C III binding sites (receptor) on non-parenchymal cells from rat liver]. 133 44

Human tooth slabs were used to observe the effect of several kinds of organic acids and proteolytic enzymes on the production of artificial caries of enamel. Acidic gels were made by formic, acetic and lactic acids separately or in mixed form. The experiments were done in a period of 10 days. After this the specimens were prepared in ground sections and observed under optic microscope. The depth of the artificial lesions was measured by a micrometric scale in the eye lens of the microscope. The enzymes used for investigation were papain, trypsin and collagenase. The surfaces of the tooth were treated with fresh enzyme solutions every day and then with mixed acid gels. This was done alternately every day in a period of ten days. The results showed that lesions produced by formic acid gel were deeper than those produced by other acids or mixed acid gels. No effect was observed on the depth of the lesion by enzymatic treatments.
Hua Xi Yi Ke Da Xue Xue Bao 1990 Sep
PMID:[The effect of several acids and proteolytic enzymes on the production of artificial caries]. 209 67

Papain, trypsin and collagenase were used in the experiment for the study of their effects on the demineralization of enamel surface by organic acids in vivo. No effect of any of the enzymes was observed. The use of papain and trypsin in treating tooth surfaces in vitro, no matter whether sodium bisulfite was used, showed no effect on the demineralization of the enamel by mixed organic acids. But, by the analysis of amino acids in the enzyme solutions before and after treating tooth surface, it was shown that some proteinous substances were destroyed. Accordingly, it can be suggested that the enzyme takes no part in the destruction of the inorganic tooth substance.
Hua Xi Yi Ke Da Xue Xue Bao 1990 Jun
PMID:[Effects of proteolytic enzymes on demineralization of enamel]. 216 74

The ultrastructural changes of infiltrating gastric carcinoma cells and their surrounding structures, including extracellular matrix and tissues of target organ were observed under electron microscope. Foot-like processes with abundant microfilaments extended from one side of the surface of cancer cell were found. The microfilaments were lost with the disappearance of foot-like processes. It hinted that there might be some relationship between the presence of microfilaments and the movement of cancer cells. Around the foot-like processes of infiltrating cancer cell, the local basal lamina always disappeared, and a zone of amorphous matrix was shown. It may result from the action of collagenase. Sometimes the basal lamina may reappear, even on the surface of metastating cancer cell. No direct evidence of destruction of smooth muscle cell by the cancer cell was found. The atrophy and degeneration of smooth muscle cells may be the changes indirectly caused by the infiltrating cancer cells.
Hua Xi Yi Ke Da Xue Xue Bao 1993 Mar
PMID:[The ultrastructural changes of infiltrating gastric carcinoma cells and their surrounding structures]. 834 85

In the present study, hypertriglyceridemia was induced by feeding S. D. rats with high carbohydrate diet (carbohydrate accounting for 80% of total calorie) for 5 days. Rat liver parenchymal cells (PC) and non-parenchymal cells (NPC) were prepared by collagenase method. Human plasma LDL, VLDL and HDL were isolated by density gradient ultracentrifugation method. 125I-LDL was labelled by ICI method. 125I-LDL binding to rat liver PC and NPC were measured by Goldstein and Brown's method. The results showed that both rat liver PC and NPC had LDL receptors to bind, uptake and degradate 125I labelled human plasma LDL. The Bmax of 125I-LDL binding to NPC was 7-fold higher than that of PC (P < 0.01), but the Kd values remained unchanged. The Bmax of 125I-LDL binding to liver NPC from hypertriglyceridemic rats was significantly higher than that of NPC from the normal diet (carbohydrate accounting for 60% of total calorie) rats (435.4 + 100.0 vs 307.1 +/- 76.8 ng/mg cell protein, n = 6, P > 0.05), but there was no difference in Kd between the HTG rats and normal rats (18.1 +/- 4.1 vs 17.2 +/- 4.0 micrograms/ml n = 6, P > 0.05). These results suggest that the increase in fractional catabolic rate of LDL in patients with hypertriglyceridemia reported by Vega and Grundy might be induced by the increase in number of LDL receptor on liver non-parenchymal cells.
Hua Xi Yi Ke Da Xue Xue Bao 1995 Sep
PMID:[Study on LDL receptors of liver non-parenchymal cells in hypertriglyceridemic rats induced by high carbohydrate diet]. 858 86

The key to preparing TILs for clinical application is to enhance its capacity of proliferation and cytotoxicity. For this purpose we have made a study of methodology and established an effective way consisting of 3 processes to separate and culture TILs. It included: (1) mechanical splitting; (2) digesting with 0.05% collagenase I and 0.003% DNAase I mixture in room temperature for 6 hrs and 18hrs in cool, and (3) centrifuging with 75% and 100% Ficoll non-continuous grade density. The separated TILs were suspended in conditional culture medium for proliferation. Most of them reached the amount of 10(9) at the end of culture, which was essential for clinical therapy. Their NK and LAK activities were 56.3 +/- 11.7% and 48.6% +/- 10.6% respectively, in which the CD6+ T cells played an important role. The results suggest this a perfect way for separating and culturing TILs.
Hua Xi Yi Ke Da Xue Xue Bao 1996 Mar
PMID:[Establishment of an effective way for separating and culturing tumor infiltrating lymphocytes]. 920 17

To explore the method of isolating acutely the smooth muscle cells from pulmonary artery in rats, small pulmonary arteries (700-200 microns, ID) were dissected free of connective tissue and were allowed to digest in a N-2-hydroxyethyl-piperazine-N'-2-ethanesulfonic acid(HEPES)-buffered physiological saline solution (HPSS) containing collagenase, papain and bovine serum albumin. The tissue was then triturated to disperse smooth muscle cells. The isolated cells in suspension were identified and photographed with film on electron microscope (EM). We succeeded in isolating the single smooth muscle cell, which appeared compressed typically. 90% cells in suspension were identified smooth muscle cells on EM. We conclude that the method for isolation of pulmonary arterial smooth muscle cells is simple, stable and effective and is recommanded for use.
Hua Xi Yi Ke Da Xue Xue Bao 1998 Sep
PMID:[Isolation and identification of smooth muscle cells from pulmonary artery in rats]. 1068 82

To investigate the behavoir of TMJ condylar cartilage cells in vitro, the mandibular condylar cartilage cells were harvested from a 5-month-old human fetus by dissection and sequential digestion with 0.25% trypsin and 0.2% collagenase (type II). The isolated cells were cultured in DMEM medium and identified by histochemical and immunohistochemical methods. Cell proliferation, morphology and ultrastructure were observed by phase-contrast microscope, cytologic staining and electronic microscope. In primarily cultured cells, polygonal chondroblast-like cells dominated and they were confirmed by the positive result of immunohistochemical examination for type II collagen and Toludin blue staining. In conclusion, the TMJ cells in this culture system kept their phenotype in vivo.
Hua Xi Kou Qiang Yi Xue Za Zhi 1997 Aug
PMID:[The culture of TMJ condylar cartilage cells and study on their biological behaviors in vitro]. 1147 91

The catalytic domains of two matrix metalloproteinases--collagenase-1 and stromelysin-1 have been studied by means of fluorescence spectroscopy and high hydrostatic pressure. The hydrophobic fluorescence probe ANS could bind to stromelysin-1, with a dissociation constant of 26.3 &mgr;mol/L, but could not bind to collagenase-1, indicating that there exists a hydrophobic site on the surface of stromelysin-1. Further study suggests that the hydrophobic site may not be the catalytic site. The biological activity of catalytic domains of collagenase-1 and stromelysin-1 showed obvious difference under high pressure the activity of collagenase-1 increased with elevating pressure, with an activation volume of D18.9 ml/mol however, the activity of stromelysin-1 did not change under high pressure. The results indicate that there are some obvious differences between the catalytic domain conformations of these two enzymes, though the crystal analysis indicated that they were very similar as reported before.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 2000
PMID:Comparison of the Catalytic Domains of Collagenase-1 and Stromelysin-1. 1207 34

This study was aimed at the feasibility of using ATP-bioluminescence assay for tumor in vitro chemosensitivity testing. With the use of this assay, the authors determined dose-response curve in mouse fibroma cell line L929 treated with chemotherapeutic agents, and investigated the different in vitro responses of 6 ovarian carcinomas (5 from fresh tumor tissues, 1 from ascites) treated with etopside, cis-plating, 5-fluorouracil and adriamycin. The results showed that the coefficients of variation for triplicate assays ranged from 1.2% to 15.8% which means high reproducibility of the assay. The single cell suspension (including < 30 cells clusters) could be separated from tissue fragments by means of enzyme cocktail (collagenase, Dnase, pronase). The viable cells were over 90%. This study demonstrates that ATP-bioluminescence assay is a sensitive, reliable and efficient method for tumor chemosensitivity testing. In this connection, the correlation between in vitro drug sensitivity and in vivo patient response is worth further studying.
Hua Xi Yi Ke Da Xue Xue Bao 2000 Sep
PMID:[An evaluation of adenosine triphosphate bioluminescence assay for tumor in vitro chemosensitivity testing]. 1254 24


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