Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.4.24.3 (collagenase)
 18,340 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

In many countries, including Brazil, extracts of Jatrophona elliptica species are currently used for the treatment of several diseases. Recently it was shown that a purified compound from these plants inhibits contraction of smooth and cardiac muscle in the microM range, probably involving alterations in membrane Ca2+ permeability and/or internal Ca2+ distribution. In collagenase-isolated rat islets and in the absence of glucose, basal insulin secretion measured by radioimmunoassay averaged 122 +/- 13 microU/islet per 90 min (N = 25). At 16.7 mM glucose, the insulin output reached 445 +/- 32 microU/islet per 90 min (N = 27). Jatrophone (1-100 microM/l) caused a dose-related inhibition of glucose-induced insulin release, over basal secretion, with an ID50 close to 8 microM/l. Complete inhibition of insulin release was obtained with 100 microM/l Jatrophone. However, at 100 microM/l (but not at 10 microM/l) concentration, Jatrophone also provoked a reduction in glucose metabolism by the islets which could explain, at least in part, the reduction in insulin secretion. After 120-min incubation, the glucose metabolism, measured by the 14CO2 production, was reduced from 26.58 +/- 3.63 (N = 42) to 7.48 +/- 1.36 (N = 16) pmol/l per islet. In conclusion, at lower concentrations (10 microM/l) Jatrophone could be a valuable tool for the study of the mechanism of insulin release induced either by glucose or other secretagogues.
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PMID:Inhibition of insulin release by Jatrophone. 134 26