Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.24.3 (
collagenase
)
18,340
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Collagenase is currently used in the isolation of rat hepatocytes, but it rapidly inactivates the heparin-releasable triacylglycerol lipase of the liver. Since
collagenase
-isolated liver cells contain a heparin-releasable
monoacylglycerol hydrolase
, a study was made on the effect of
collagenase
treatment on the substrate specificity of purified heparin-releasable lipase of rat liver. Incubation of the purified lipase with
collagenase
selectively decreased the triacylglycerol lipase activity of the enzyme with no effect on the
monoacylglycerol hydrolase
activity. Gel filtration of the lipase before and after
collagenase
treatment indicated cleavage of a small molecular weight fragment from the enzyme. This resulted in a preparation with less triacylglycerol lipase activity but still capable of monoacylglycerol hydrolysis.
...
PMID:Modification of the substrate specificity of rat hepatic lipase by collagenase treatment. 22 27
