Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.4.24.3 (collagenase)
 18,340 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

In order to study human fat cell morphology and metabolism in both sexes, with different levels of fatness and physical training, 53 subjects (14 women and 39 men), 25.9 +/- 7.6 (mean +/- SD) years of age, weighing 64.8 +/- 10.8 kg and with 14.8 +/- 4.7% body fat were submitted to a biopsy of subcutaneous adipose tissue in the supra-iliac region. Average fat cell diameter (ACD) was assessed by measuring at least 500 cells per subject. Basal (BL), epinephrine sub-maximal (10(-5) M) (ESML) and epinephrine maximal (10(-4) M) (EML) stimulated lipolysis have been determined on collagenase isolated fat cells. Female subjects have larger ACD (83.4 +/- 12.3 micrometer vs. 62.5 +/- 11.9 micrometer) (P less than or equal to 0.001) and lower BL, ESML, and EML than men (P less than or equal to 0.05). After control over age and sex of subjects, there were significant and negative correlations between all fatness indicators (ACD, fat mass in kg, percent body fat, sum of 9 skinfolds) and BL, ESML and EML. A completely randomized factorial ANOVA design revealed a significant effect of fatness level on BL, ESML and EML, but a non-significant trend for the effect of physical training on the same variables. It is concluded that lipolytic activity is more related to body fatness than to the training status. However, training tends to accentuate lipolytic activity as shown by values of BL, ESML and EML found in the lean highly trained subjects of the study.
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PMID:[Morphology and metabolism of human adipose tissues: relation to sex, body fat and level of training]. 619 Apr 51

In order to study the effects of heredity and of physical training on adipose tissue morphology and metabolism, 15 pairs of monozygotic twins (MZ) (six males and nine females), aged from 16 to 24 years, weighing 56.2 +/- 9.1 kg and with 13.9 +/- 8.2 percent body fat, were submitted to a biopsy of adipose tissue in the suprailiac region. In addition, eight pairs of twins (four male and four female) took part in a 20-week ergocycle training program, five days a week, 40 min a day, at 80 percent of their maximal heart rate. Adipocyte diameter (AD) was assessed on collagenase isolated fat cells. Basal (BL), epinephrine submaximal (10(-5) M) (ESML) and epinephrine maximal stimulated lipolysis (10(-4) M) (EML) were determined on isolated fat cells. Intraclass correlation coefficients indicated significant intrapair resemblance before training for all fat morphological and metabolic indicators (0.78 less than or equal to ri less than or equal to 0.93). Training significantly increased VO2 max (pre: 44.7 +/- 7.6 (ml/kg) vs post: 50.8 +/- 5.0; P less than or equal to 0.001). No training effect was found in percent body fat and AD. Training significantly increased BL, ESML, and EML (P less than or equal to 0.01). Moreover, twins of the same MZ pair yielded identical responses in ESML and EML with training. Intraclass coefficients for the magnitude of change in activity over pretraining values reached 0.84 and 0.90 respectively. Apparently a genetically determined response to training could not be found for BL. These results show that training per se has an effect on adipose tissue lipolysis beyond variation in fatness. Furthermore, sensitivity of stimulated lipolysis to training appears to have a genetic basis.
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PMID:Adaptive changes to training in adipose tissue lipolysis are genotype dependent. 653 62