Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.24.3 (
collagenase
)
18,340
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Skin circadian clock system responds to daily changes, thereby regulating skin functions. Exposure of the skin to UV irradiation induces the expression of
matrix metalloproteinase-1
(
MMP-1
) and causes DNA damage. It has been reported both DNA repair and DNA replication are regulated by the circadian clock in mouse skin. However, the molecular link between circadian clock and
MMP-1
has little been investigated. We found PERIOD protein, a morning clock component, represses the expression of
MMP-1
in human keratinocytes by using a
PER
-knockdown strategy. Treatment with siPer3 alleviated the suppression of
MMP-1
expression induced by forskolin. Results revealed PER3 suppresses the expression of
MMP-1
via cAMP signaling pathway. Additionally, we screened for an activator of
PER
that could repress the expression of
MMP-1
using HaCaT cell line containing
PER
promoter-luciferase reporter gene. Results showed
Lespedeza capitate
extract (LCE) increased
PER
promoter activity. LCE inhibited the expression of
MMP-1
and its effect of LCE was abolished in knockdown of PER2 or PER3, demonstrating LCE can repress the expression of
MMP-1
through
PER
. Since circadian clock component
PER
can regulate
MMP-1
expression, it might be a new molecular mechanism to develop therapeutics to alleviate skin aging and skin cancer.
...
PMID:PER, a Circadian Clock Component, Mediates the Suppression of MMP-1 Expression in HaCaT Keratinocytes by cAMP. 2957 Jun 74
