Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.4.24.3 (collagenase)
 18,340 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Storage of pancreatic islets by cryopreservation would greatly facilitate a large scale program of clinical islet transplantation. We report success on long-term follow-up with autotransplantation of frozen/thawed canine pancreatic fragments. Total pancreatectomy and islet isolation by collagenase ductal perfusion and mechanical disruption preceded either acute autotransplantation or cryogenic preservation prior to autotransplantation. Cryopreservation was by dimethylsulfoxide equilibration, cooling at 0.25 degrees C/min to -75 degrees C, storage in liquid N2 and thawing at 3.5 degrees C/min. Four of five acutely autotransplanted dogs remained normoglycemic for 20 months, with three of four maintaining normal K values on intravenous glucose tolerance test (IVGTT) and nondiabetic values on oral GTT. Four of four dogs transplanted with frozen/thawed islets remained normoglycemic for 15 months with three of four maintaining nondiabetic IV GTT K values and normal oral GTTs for 15 months. Both acutely transplanted and frozen/thawed islets are capable of maintaining long-term metabolic control. Cryopreservation preserved viability of sufficient canine pancreatic islets to reverse diabetes with autotransplantation. Function of the frozen-thawed islets showed minimal deterioration during a follow-up of 15 to 18 months.
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PMID:Long-term normoglycemia in pancreatectomized dogs transplanted with frozen/thawed pancreatic islets. 308 86

A total of 116 adult male and female rats of own inbred F3 up to 8th generation weighing 180-350 g was rendered diabetic by a single i. v. injection of either alloxan (40-50 mg kg-1) or streptozotocin (50-60 mg kg-1) and 1--2 weeks later subjected to the intrafamiliar homo(allo)transplantation of 2--5 minced, collagenase non digested pancreases either from own newborns or from newborns of sisters and brothers of the same family under the renal capsule. Out of the total, 24 animals (20.68%) were successfully cured. The cured rats examined with the aid of intravenous glucose tolerance test (IV) GTT monthly for one year showed fasting normonoglycemia with increased postglucose values and decreased KG being in the range of latent, borderline diabetes. In male rats IV GTT deteriorated in nine months without any transition into manifest diabetes or loss of body weight. Some animals cured by transplantation reached a normal survival time and were able to bring forth litters and suckle their newborns. In the rats with complete reversal of diabetes the transplants with well developed, vascularized islets, consisting predominantly of B-cells were found.
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PMID:Treatment of alloxan and streptozotocin diabetes in rats by intrafamiliar homo (allo) transplantation of neonatal pancreases. 644 61

It is known that renin is present in fetal membranes, with the highest concentration in the chorion laeve (reflected chorion). The purpose of this study was to identify and localize renin in human chorion laeve. Indirect immunofluorescent analysis, using antiserum against pure human kidney renin, revealed a single layer of cells in the chorion with strongly positive fluorescence. The presence of atrophic villi in this layer together with other morphological evidence indicate that the cells which are positive for renin are cytotrophoblasts. Isolated cells were prepared from the chorion by collagenase digestion, followed by filtration and density gradient centrifugation on Percoll. The isolated cells also showed a positive reaction with the immunofluorescent technique. Control experiments with nonimmune serum did not show fluorescent cells. Biochemical analysis using RIA of angiotensin I generated from sheep substrate indicated that most of the renin activity in the isolated cells was present as inactive renin (activated by trypsin). The presence of renin in trophoblastic cells may be of significance in local cardiovascular regulation, events associated with parturition, or pathophysiological manifestations of trophoblastic disease.
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PMID:Localization of renin in trophoblasts in human chorion laeve at term pregnancy. 702 20