Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.4.24.3 (collagenase)
 18,340 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

It was decided to study the effect of glucose deprivation on collagen synthesis and degradation in fibroblast cultures and a correlation of these processes with the expression of oxygen/glucose regulated proteins (ORP150/GRP170). The incorporation of radiolabeled proline into collagenase-sensitive and hydroxyproline-containing proteins was used as an index of collagen synthesis, whereas pulse-chase technique was employed to evaluate the degradation of newly synthesised proteins. We demonstrated that fibroblasts incubated in high-glucose medium synthesised detectable amounts of collagenous proteins. Most of them were secreted into the culture medium. The shortage of glucose resulted in about 30% reduction in synthesis of collagenous proteins, both those secreted into culture medium and remaining in the cell layer. The pulse-chase experiments demonstrated that the reduced amount of newly synthesised collagen was protected against intracellular degradation. Proportionally less collagen was degraded in cultures incubated in low-glucose than in high-glucose media. These phenomena were accompanied by an increase in the expression of chaperon-ORP150 in cultures growing in low-glucose medium. We suggest that the increased expression of ORP150 is a factor which protects collagen against intracellular degradation induced by glucose deprivation.
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PMID:The effect of glucose deprivation on collagen synthesis in fibroblast cultures. 1922 68