Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.4.24.3 (collagenase)
 18,340 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

DNA synthesis, measured as 3H-thymidine incorporation, was estimated in islets obtained from BB rats before the onset of diabetes and compared with the replication rate of islets obtained from age-matched BB rats having never developed hyperglycaemia. Using a biopsy procedure, splenic pancreas was removed from both 65 and from 80 day old diabetes prone BB rats. After isolation by a modified collagenase method pancreatic islets were incubated with labeled thymidine. The animals submitted to biopsy were followed up until they reached an age of 200 days. The diabetes incidence of the BB rats biopsied at day 65 and at day 80 was comparable (35% and 31%). In both groups approximately 140 mg of splenic pancreas were necessary to isolate 70-90 islets, regardless whether the animals become diabetic or not. Using a retrospective experimental design, the thymidine incorporation into islets obtained from prediabetic (rats which developed hyperglycaemia) and from normoglycaemic BB rats were comparatively analysed. The DNA synthesis of pancreatic islets obtained from 65 day old prediabetic BB rats is not different from that measured in islets from normoglycaemic BB rats. A similar replicatory behaviour was also observed using islets from prediabetic and normoglycaemic 80 day old BB rats. From our results we seek to conclude that a prediabetic state is not related to a diminished replicatory activity of pancreatic islets.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:3H-thymidine incorporation into islets obtained from prediabetic BB rats. 267 17

The author describes his findings pertaining to spontaneous diabetes mellitus in BB rats and the method and results in juvenile alloxan diabetes in neonatal and adolescent Wistar rats of his own inbreeding F8-10. The author presents also the results of attempts to treat juvenile alloxan diabetes in rats by intrafamilial renal-subscapular allotransplants of 2-5 neonatal collagenase nondigested pancreases. Six of eleven BB females developed latent or manifest insulin dependent diabetes mellitus during the third to fourth month of life. An intraperitoneal injection of alloxan to 2-5-day-old rats causes, after two months of prediabetes, latent or manifest disease, in particular in males. In one-two-month adolescent fasting F6--10 inbred rats (Wistar strain) intravenous injection of 50 mg/kg alloxan causes diabetes mellitus with hyperglycaemia (20-60 mmol/l), glycosuria, polyuria, arrested growth, development of cataract and early death due to pulmonary or intestinal infection. The author tries to prevent these sequelae and complications by insulin therapy or intrafamilial allotransplantations of 2-5 neonatal, collagenase nondigested pancreases beneath the renal capsule, using two-three--week immunosuppression with Cyclosporin A combined with Azathioprine. The author proves permanent cure, histologically and functionally, by repeated allotransplantation which, however, due to the intense thymolymphatic immunological barrier in adolescent rats is less frequent than cure repeatedly achieved by the author in adult diabetic rats.
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PMID:[Spontaneous and experimental models of human juvenile diabetes mellitus]. 275 53