Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.4.24.3 (collagenase)
 18,340 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

1. Adult rat skeletal muscles were dissociated by collagenase treatment and trituration, and the isolated muscle fibres were maintained in vitro for 2-3 weeks. At various stages, the fibres were examined physiologically and morphologically. 2. The isolated fibres underwent some changes characteristic of muscle denervated in vivo. For instance, input resistance increased and extrajunctional acetylcholine (ACh) receptors appeared. In addition, the beginning stages of apparent muscle fibre fragmentation were observed. 3. In other respects, the cultured isolated fibres behaved differently than in vivo denervated fibres. Fibrillation developed only occasionally in vitro. The onset of ACh supersensitivity was slower (6 days) than after denervation in vivo (2-3 days). Some fibres developed localized regions of destriation, which apparently was due to loss of in-register alignment of myofibrils.
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PMID:Properties of isolated adult rat muscle fibres maintained in tissue culture. 92 4

Fibrillation of articular surface and depletion of proteoglycans are the structural changes related to early osteoarthrosis. These changes make cartilage softer and prone to further degeneration. The aim of the present study was to combine mechanical and acoustic measurements towards quantitative arthroscopic evaluation of cartilage quality. The performance of the novel ultrasound indentation instrument was tested with elastomers and bovine articular cartilage in vitro. The instrument was capable of measuring elastomer thickness (r = 1.000, p < 0.01, n = 8) and dynamic modulus (r = 0.994, p < 0.01, n = 13) reliably. Osteochondral plugs were tested before and after enzymatic degradation of cartilage proteoglycans by trypsin or chondroitinase ABC, and of cartilage collagens by collagenase. Trypsin and collagenase induced a mean decrease of -31.2 +/- 12.3% (+/- SD, p < 0.05) and -22.9 +/- 20.8% (p = 0.08) in dynamic modulus, respectively. Rate of cartilage deformation, i.e. creep rate, increased by +117.8 +/- 71.4% (p < 0.05) and +24.7 +/- 35.1% (p = 0.17) in trypsin and chondroitinase ABC treatments, respectively. Collagenase induced a greater decrease in the ultrasound reflection from the cartilage surface (-54.2 +/- 29.6%, p < 0.05) than trypsin (-17.1 +/- 13.5%, p = 0.08). In conclusion, combined quantitation of tissue modulus, viscoelasticity and ultrasound reflection from the cartilage surface provides a sensitive method to distinguish between normal and degenerated cartilage, and even to discern proteoglycan loss and collagen degradation from each other.
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PMID:Novel mechano-acoustic technique and instrument for diagnosis of cartilage degeneration. 1221 58