Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.24.3 (
collagenase
)
18,340
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Multiple symmetric lipomatosis
(
MSL
) is characterized by enlarging, painless fat deposits in the neck and upper trunk. The pathogenesis of
MSL
is unknown. Owing to localization of
MSL
fat deposits in the neck and interscapular region, it has been suggested that they could originate from brown fat. However, the histological appearance of
MSL
adipose tissue is that of white fat, with prevailing monovacuolar adipocytes. Nevertheless,
MSL
adipocytes are smaller than adipocytes of the common white adipose tissue and show peculiar metabolic features. The ultrastructure of
MSL
lesions has been not described. The present work investigated the ultrastructural morphology of
MSL
adipose tissue and lipomatous adipocyte precursors maintained in long-term culture. Samples of lipomatous tissue were obtained from patients affected with
MSL
undergoing surgical lipectomy. Portion of the tissue was processed for electron microscopy; the rest was digested with
collagenase
, and isolated preadipocytes from the stromal-vascular fraction were cultured up to 15 days. Cultured cells were prepared for electron microscopy in situ and their morphology compared with human white adipose tissue preadipocytes and rat brown preadipocytes cultured in parallel. Results show the following. 1) Adipocytes of
MSL
are not monovacuolar and resemble the largest adipocytes that can be found in rat and human brown fat. 2) Some morphological features of
MSL
adipocyte precursors resemble brown adipocyte more than white: cultured
MSL
preadipocytes transiently develop large mitochondria with parallel cristae resembling those of the brown fat cell and maintain a multivacuolar lipid deposit in culture, i.e. a typical feature of brown preadipocytes. 3) Some morphological features suggest a neoplastic nature of
MSL
adipocytes. Taken together, these findings suggest that
MSL
is a neoplastic disease which could originate in brown fat.
...
PMID:Multiple symmetric lipomatosis. Ultrastructural investigation of the tissue and preadipocytes in primary culture. 238 Nov 66
