Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts: Gene/Protein Disease Symptom Drug Enzyme Compound   Target Concepts: Gene/Protein Disease Symptom Drug Enzyme Compound

---

Query: EC:3.4.24.3 (collagenase)
18,340 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Equine immunoglobulin was detected along the glomerular basement membrane of three human homograft recipients who had been treated with equine anti-lymphocyte globulin. Anti-lymphocyte globulins, given these patients, were obtained by immunization of horses with lymphocytes from human spleens and/or lymph nodes and contained glomerular basement membrane-reactive antibodies. Quantitative paired-label isotope experiments (in rats) demonstrated that 30-170 mug/ml of kidney-fixing antibodies were present in these preparations. The anti-lymphocyte globulins formed a line of identity with a sheep anti-human glomerular basement serum when reacted against collagenase-solubilized human glomerular basement membrane in double diffusion in agar. The renal fixation of these antibodies was blocked by absorption with human glomerular basement membrane, but not by buffy-coat leukocytes, indicating that they were directed specifically toward antigens in the basement membrane and were not cross-reacting anti-lymphocyte antibodies. Anti-lymphocyte globulin preparations for human use were studied for glomerular basement membrane-reactive antibodies by a direct immunofluorescent assay in rats. Anti-lymphocyte globulin from 13 of 20 horses, and 7 of 10 serum pools from horses immunized with lymphocytes derived from solid lymphoid organs (spleen, thymus, lymph node, tonsil), contained glomerular basement membrane-reactive antibodies. Sera from 18 horses injected with thoracic duct cells or cultured lymphoblasts had no glomerular basement membrane-reactive antibodies. An equine anti-human thymus serum containing glomerular basement membrane-reactive antibodies, which produced fatal glomerulonephritis in monkeys, was shown to cause both immediate and delayed glomerular injury in monkeys after intravenous injection. The reaction of this antibody with glomerular basement membrane in vivo was associated with little complement deposition in spite of the fact that the antibody could fix complement. This lack of glomerular complement fixation resulted from almost complete in vivo decomplementation of the monkeys receiving this anti-lymphocyte globulin.
...
PMID:Glomerular basement membrane--reactive antibody in anti-lymphocyte globulin. 499 86

Components were solubilized from human glomerular basement membrane by digestion with collagenase and pepsin or by extraction with guanidine-HCl either directly or after previous digestion with the enzyme. The diverse preparations were used as antigens in the enzyme-linked immunosorbent assay (ELISA) of antibody titers in sera from patients with Goodpasture syndrome and patients with other forms of glomerulonephritis, that is, systemic lupus erythematosus, periarteritis nodosa, and IgA-related nephropathy. Patients with Goodpasture syndrome had high titers of IgG antibodies reacting most strongly with collagenase digests. The antigen(s) was only partly solubilized by guanidine-HCl extraction, was destroyed by pepsin digestion as well as reduction, and partly destroyed by trypsin digestion. The antigen(s) is most likely noncollagenous protein. Antibodies from patients with other forms of nephritis were directed primarily against antigens in guanidine-HCl extracts, while the antigen(s) was not solubilized by collagenase digestion. Pepsin digestion destroyed the antigen(s). The antibodies were of a different class, that is, the patients with systemic lupus erythematosus had IgG and IgA as well as IgM antibodies; the patients with periarteritis nodosa had IgM or IgG and IgA antibodies, while the patients with IgA-related nephritis had the highest recorded titers of IgA but also had IgG as well as IgM antibodies. None of the patients had antibodies directed against triple helical collagen. The antibody response in anti-GBM antibody-related nephritis, then, is different both with respect to antigen and antibody class and depends on the underlying disease syndrome.
...
PMID:Antiglomerular basement membrane antibody: antibody specificity in different forms of glomerulonephritis. 613 25

Leukocyte adherence inhibition (LAI) test was examined in 35 patients with renal diseases and 14 normal controls, using collagenase-treated glomerular basement membrane, glycosidase-treated glomerular basement membrane and renal tubular epithelium as antigens. Although the control group showed strikingly similar mean LAI indices for all antigens tested, the whole group of patients with renal diseases showed a wide scatter of values. Two categories of patients had significantly increased LAI indices (p less than 0.01) when their mean values were compared with those of normal controls: (1) rapidly progressive glomerulonephritis (RPGN) and (2) lupus nephritis (SLE). In the serial studies of the RPGN and SLE cases, there were no significant changes in the pattern of LAI and they continued to give positive and very comparable results when re-examined at intervals of 1-6 months. Out of the 30 patients who were able to be evaluated with the three antigens, 15 cases exhibited positive LAI response to two or more antigens simultaneously. These in vitro findings suggest that there is an abnormal cellular response to certain antigen or widespread LAI reactivity to a variety of renal antigens in certain forms of human glomerulonephritis.
...
PMID:Leukocyte adherence inhibition test in renal diseases. 617 83

Preparations of human glomerular basement membrane (GBM) were digested with collagenase, and a Goodpasture (GP) antigen rich pool from gel filtration column runs was identified by antibody inhibition radioimmunoassay. The components of the GP antigen pool were separated on polyacrylamide gels, and transferred to nitrocellulose sheets by the 'western' blotting technique. The blots were separately reacted with thirteen GP sera as primary antibody, followed by peroxidase labelled goat anti-human IgG and revealed 45-50K (two bands) and 25-28K (one-three bands) components. No corresponding reactivity was observed using convalescent GP sera or other control sera (normal human serum, rapidly progressive glomerulonephritis with or without pulmonary haemorrhage, and lupus erythematosus) as primary antibody.
...
PMID:Detection of Goodpasture antigen in fractions prepared from collagenase digests of human glomerular basement membrane. 631 59

The antigen involved in the glomerulonephritis associated with antibodies to glomerular basement membrane (GBM) was purified from human GBM digested with highly purified clostridial collagenase. The purified nonreduced sample contained two components with closely similar mobilities on sodium dodecyl sulfate/polyacrylamide gel electrophoresis. After reduction they moved as one, nonantigenic, component, corresponding to a molecular weight of 26,000. Immunologically identical aggregates of higher molecular weight (i.e., 48,000) were also identified in the crude digest. Reduction of such aggregates after purification released some protein with a molecular weight of 26,000, but a large proportion was insensitive to reduction. Seven patients with Goodpasture syndrome all had circulating anti-GBM antibodies directed only against the purified antigen.
...
PMID:Isolation of the specific glomerular basement membrane antigen involved in Goodpasture syndrome. 632 1

A fluid phase radioimmunoassay (RIA) has been established for the detection of circulating anti-glomerular basement membrane (GBM) antibodies. Antibodies were detected by binding with a collagenase solubilised, immunochemically purified, radiolabelled extract of human particulate GMB. Sera from 108 patients with a variety of histological types of glomerulonephritis (GN) were assessed for the presence of antibodies. Likely positive sera were those from patients with clinical features of rapidly progressive glomerulonephritis (RPGN) with crescentic or diffusely proliferative GN and linear staining of their GBM's with IgG. Thirty one of 35 sera from such patients were positive in the RIA. Sera from 14 patients with RPGN but with granular IgG deposition (i.e. immune complex related GN) were all negative for anti-GBM antibodies as were sera from 59 patients with a variety of non-crescentic non RPGN without linear IgG. Comparison of the RIA with conventional indirect immunofluorescence (IIF) showed that the RIA had a higher detection rate of antibodies in likely positive sera (19/21 RIA: 16/21 IIF) lower false positive detection rate with sera from patients without GN (0/26 RIA: 2/26 IIF) and was three to four times more sensitive in serial dilution studies.
...
PMID:A radioimmunoassay for the detection of circulating anti-glomerular basement membrane antibodies. 634

An indirect haemagglutination (IHA) test using tanned sheep erythrocytes was developed to detect antibodies in sera from rabbits immunized with renal tubular epithelium (RTE) or with glomerular basement membrane (GBM) from rats. The RTE antigen preparation, called Fx1A, sensitized the erythrocytes directly, whereas the GBM preparation had to be treated with collagenase. THe antisera to Fx1A gave titres up to 64,000, and the antisera to GBM up to several million. The sera were monospecific, since they gave no cross-reactions in IHA, precipitation, or absorption tests. Using the IHA test, the quantitative aspects of experimental glomerulonephritis in rats can be studied. Preliminary results obtained in transfer experiments indicate a dose-response relationship between the titres in IHA and the degree of proteinuria.
...
PMID:Indirect haemagglutination for the demonstration of antibodies to renal antigens from rats. 701 79

Anti-basement membrane antibodies are now being associated with an increasing spectrum of disease, including Goodpasture's syndrome, rapidly progressive and occasionally milder forms of glomerulonephritis (GN), tubulointerstitial nephritis, pulmonary damage, and potentially other forms of tissue injury. We have developed a radioimmunoassay to detect circulating antiglomerular basement membrane (GBM) antibodies. The antigens for this assay are derived from the noncollagenous portion of the GBM remaining after collagenase digestion. After immunoabsorptive purification, the major antigens precipitated by human anti-GBM antibodies can be characterized by polyacrylamide gel electrophoresis (PAGE) into an unresolved high molecular weight fraction and two antigenic peaks of 54,000 and 27,000 daltons. The noncollagenous nature of the antigenic material has been confirmed by amino acid analysis. The radiolabelled antigen has proven useful in detecting circulating anti-GBM antibodies in over 500 patients. The assay is of use in monitoring the activity of disease and judging the patient's response to therapy. It is also useful in determining the timing of renal transplantation, if required. Differences in antigenic content of glomerular and tubular basement membranes (TBM) have been noted between individuals. These antigenic differences, under certain circumstances, can lead to the induction of anti-basement membrane antibody responses after transplantation.
...
PMID:Anti-basement membrane antibodies in immunologic renal disease. 702 Jun 72

Glomerular basement membranes (GBM) were isolated and subjected to enzymatic degradation with the protease trypsin (Serva), chymotrypsin (Serva), papain (Sigma), pepsin (Serva) and collagenase (Worthington) as well as a lysosomal preparation from glass adherent rat blood and peritoneal exudate cells. Split products were characterized by immunoelectrophoresis and cellulose acetate electrophoresis. Urine was obtained from healthy rats and rats with Masugi's experimental glomerulonephritis, dialyzed and concentrated and applied on immunoelectrophoresis, using anti-GBM antibody from rabbit. Urinary GBM split products from healthy and nephrotic rats showed two precipitation lines like digestion products obtained after chymotrypsin degradation. This finding was supported by characterizing individual antigenic degradation products obtained after inhibition of GBM degradation by the lysosomal preparation with ethylenediaminetetraacetic acid and Trasylol alone and in combination, as well as with o-phenanthrolin. It is concluded that GBM-antigens excreted into urine indicate limited digestion of GBM by chymotrypsin-type protease.
...
PMID:Proteolytic degradation of the glomerular basement membrane and immunochemical characterization of split products. 703 90

The Goodpasture's epitope has been mapped to the alpha 3 non-collagenous chain (NC1) of type (IV) collagen [alpha 3col(IV)]. We have developed a model of experimental autoimmune glomerulonephritis (EAG) in rats immunized once with collagenase solubilized GBM (csGBM). Engelbreth-Holm-Swarm (EHS) tumor contains abundant col(IV) with little or no alpha 3col(IV). To test the hypothesis that antigens related to Goodpasture epitope are required to produce EAG in our model, we immunized rats once with 40 micrograms csEHS. Positive controls immunized with csGBM developed typical EAG with GBM bound antibody, proteinuria, and glomerulonephritis. EHS rats developed circulating and bound antibody to mesangium and tubular basement membrane with minimal GBM deposits, but did not develop proteinuria or glomerulonephritis. Although circulating antibody in EHS rats bound to csGBM by ELISA, there was no binding in ELISA to M2 antigen containing the Goodpasture epitope while EAG rat's serum did bind. By Western blot with antisera to Goodpasture epitope, EHS antigen was less complex than GBM in the monomer/dimer regions and appeared to lack NC1 corresponding to alpha 3col(IV). Blotting with sera from EHS rats demonstrated reactivity to various components of GBM but not to alpha 3col(IV). EAG sera and renal eluates bound to alpha 3col(IV). EAG rats evidenced cell mediated immunity while EHS rats did not (stimulation index EHS 1.1, EAG rats 8.0).(ABSTRACT TRUNCATED AT 250 WORDS)
...
PMID:Study of EHS type IV collagen lacking Goodpasture's epitope in glomerulonephritis in rats. 753 54


<< Previous 1 2 3 4 5 6 Next >>

---