Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.4.24.3 (collagenase)
 18,340 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A method is described for the isolation of parietal cells from the gastric mucosa of the guinea pig by enzymatic digestion with collagenase. A suspension was obtained that contained 70-80% parietal cells. About 80% of the cells were viable immediately after incubation, but viability dropped sharply after one hour. Parietal cells were identified by their morphology on light and electron microscopy, by their uptake of neutral red, by immunofluorescent staining and by carbonic anhydrase activity. Antibodies to four distinct parietal-cell antigens were obtained from rabbits immunized with the isolated parietal cells or fractions thereof. These antibodies were directed against the microsomal fraction of the parietal-cell cytoplasm, the plasma and nuclear membranes, the soluble proteins, and Castle's intrinsic factor. The antibody against the microsomal fraction, though reacting in the same way as the antibody to parietal cell canaliculi found in the serum of patients with pernicious anaemia, showed greater species specificity.
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PMID:Isolation of parietal cells from guinea-pig gastric mucosa and the immunological characterization of their antigenic structure. 5 72

The localization of antigens reacting with parietal cell antibodies found in the sera of some patients with pernicious anaemia was studied by the indirect immunoperoxidase method on viable gastric glands isolated from the mouse or human fundus by collagenase digestion. We observed that only the luminal surface of parietal cell bound antibodies, whereas their lateral and basal surfaces were not stained. From this data it is concluded that parietal cell antibodies are unlikely to be cytotoxic in vivo.
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PMID:Parietal cell autoantibodies: localization of their binding sites on isolated gastric glands. 634 80