Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.4.24.27 (thermolysin)
 1,894 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The immunomodulating effects of the A fragment of recombinant human interferon-alpha 2 (rIFN-alpha 2) generated by thermolysin treatment was compared to that of the intact rIFN-alpha 2 and lymphoblastoid IFN. The A fragment was similar to intact IFN in augmenting natural killer (NK) activity and suppressing the proliferation of certain cell lines, but it failed to inhibit human polyclonal immunoglobulin production; rIFN-alpha 2 and lymphoblastoid IFN were equally effective in suppressing this in vitro system. These data suggest that the A fragment mediates certain, but not all, of the immunomodulating properties of IFN-alpha 2.
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PMID:Immunological activities of recombinant interferon-alpha 2 and its A fragment. 361 42

To attempt to locate functionally important regions of the interferon (IFN) molecule, recombinant human IFN-alpha 2 was subjected to proteolytic digestion. The bacterial proteinase thermolysin produced two major complementary fragments, HuIFN-alpha 2-(1-110) and HuIFN-alpha 2-(111-153). After reduction with 2-mercaptoethanol and separation of the two major fragments on NaDodSO4/polyacrylamide gel electrophoresis, antiviral activity persisted in the larger, Mr 12,000, fragment consisting of the amino-terminal 110 amino acids.
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PMID:Biologic activity in a fragment of recombinant human interferon alpha. 619 90