Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.24.27 (
thermolysin
)
1,894
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Kallikrein 7
(
hK7
), a chymostatin-like serine protease, is overexpressed in pancreatic adenocarcinomas as well as other human cancers. Although it has been demonstrated to participate in normal desquamation by facilitating cell shedding at the skin surface, its role in human malignancies remains unclear. To investigate the ability of
hK7
to degrade components of the extracellular matrix (ECM), recombinant
hK7
was expressed and purified from cultured mammalian cells. Using a three-step chromatographic purification procedure, recombinant
hK7
was obtained that displayed robust proteolytic activity against a fluorogenic peptide substrate following activation by
thermolysin
. We demonstrate that the active protease is able to cleave fibronectin in a time-dependent manner, but not laminin, using an in vitro degradation assay. These findings indicate that the aberrant expression and secretion of
hK7
in human tumors may facilitate metastasis by directly degrading components of the extracellular matrix and may thus play an important role in tumorigenesis.
...
PMID:The extracellular matrix protein fibronectin is a substrate for kallikrein 7. 1834 20
