Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.24.27 (
thermolysin
)
1,894
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Limited proteolysis of buffalo plasma fibronectin (FN) by
thermolysin
yielded four gelatin-binding fragments of which, the major 59 kDa fragment,
GBF1
, was isolated by gelatin-Sepharose and heparin-Sepharose affinity columns.
GBF1
appeared during early phase of
thermolysin
digestion and remained intact even after 4 hr of digestion.
GBF1
may be similar to 56 kDa gelatin-binding fragment of FNs from human and hamster plasma. But, it is more resistant to
thermolysin
cleavage. The fragment binds to heparin with low affinity. On the basis of the structure of human plasma FN, the modular structure of
GBF1
may be given as: 6Fn1 1Fn2 2Fn2 7Fn1 8Fn1 9Fn1 1Fn3. Biophysical properties of
GBF1
suggest an expanded native conformation. The interaction of the fragment with gelatin is pH-dependent and independent of NaCl concentration.
...
PMID:Characterization of a 59 kDa gelatin-binding fragment of buffalo plasma fibronectin. 2289 98
