Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.24.27 (
thermolysin
)
1,894
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The use of polyelectrolytes is a prospective approach to form nanocomplexes to transport different compounds including proteins. In many cases, the bound protein should be digested after delivery to the target. In the present work, we studied proteolysis of
glyceraldehyde-3-phosphate dehydrogenase
(
GAPDH
) in the complexes with polyelectrolytes. We have found polyanions to enhance the proteolytic degradation of
GAPDH
by proteinase K and
thermolysin
. This effect seems to be caused by destabilization of the protein structure. However, this destabilization is reversible since the release of the enzyme from the complexes with polymers (even tightly bound with the protein such as sulfated polymers and supercharged pyridinium polycations) was accompanied by partial or complete reactivation of
GAPDH
, depending on the polymers and conditions. Finally, we observed that complexation with sulfated polymers enhances the proteolytic degradation of prion fibrils by proteinase K. The obtained results can be useful for treatment of pathologies associated with amyloid aggregation.
...
PMID:Tightly bound polyelectrolytes enhance enzyme proteolysis and destroy amyloid aggregates. 2970 11
