Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.4.24.27 (thermolysin)
 1,894 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Bovine beta-lactoglobulin variant B was hydrolysed with thermolysin at various concentrations of calcium ions ranging between 0 and 50 mM. The changes in calcium concentration did not influence the overall rates of beta-lactoglobulin proteolysis. However, HPLC analyses of the hydrolysates, obtained at nine different calcium concentrations, indicated that the rates of production and/or lysis of 3 of 17 identified peptides were calcium concentration-dependent. Proteolysis at low calcium concentrations yielded peptides V41-E45 and V43-L57. The peptide V43-L57, containing a cluster of dicarboxylic amino acids, was resistant to further hydrolysis by thermolysin even after 10 h incubation. An increase in calcium concentration triggered gradual hydrolysis of this peptide. Its hydrolytic product (peptide V43-D53) was obtained only by proteolysis of beta-lactoglobulin with thermolysin at high calcium concentration.
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PMID:Limited proteolysis of beta-lactoglobulin using thermolysin. Effects of calcium on the outcome of proteolysis. 818 Jan 43