Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.24.27 (
thermolysin
)
1,894
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Condensation
of Cbz-Asp and PheOMe catalyzed by a neutral protease from Vibrio sp. T1800 (Vimelysin: VLN) was studied. VLN showed a relatively higher catalytic activity of condensation and an apparently larger yield after 3 h or 24 h, in comparison with
thermolysin
(
TLN
), especially at lower pH and temperatures.VLN showed higher solvent-tolerance than
TLN
. TLhe apparent highest yield (25%) was obtained in 30% DMSO by using VLN; under similar conditions,
TLN
gave only about a half of this value. The rate of the condensation reaction per mole of enzyme (v/[E](o)) in DMSO 50% at 37 degrees C and pH 6.5 was 0.16 s(-1) for VLN and 0.047 s(-1) for
TLN
. In 30% ethanol VLN showed more than three-fold peptide yield than
TLN
after 5 h reaction. (c) 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 53: 387-390, 1997.
...
PMID:Peptide condensation activity of a neutral protease from Vibrio sp. T1800 (Vimelysin). 1863 27
