Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.24.23 (
MMP
)
4,246
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Purpose:
N-myc downstream-regulated gene 2
(
NDRG2
) is frequently down-regulated in cancer, and plays an important role in the control of tumor growth and metastasis. Its manipulation has been suggested as a therapy in cancer. Here, we examined the outcome of
NDRG2
overexpression on proliferation, invasion, migration and
MMP
activity of HCT116 colorectal cancer cell line.
Methods:
The HCT116 cell line (human colorectal cancer) was transfected with pCMV6-AC-GFP-
NDRG2
. 2,5diphenyltetrazolium bromide (MTT) assay was used to detect cell proliferation. The invasion and migration of the transfected cells were examined through transwell chambers while the MMP-9 activity was detected by the ability of the cells to digest gelatin.
Results:
Overexpression of
NDRG2
by stable
NDRG2
transfection decreased cell proliferation, migration and invasion ability, along with decreasing MMP-9 activity.
Conclusion:
Our data indicate that
NDRG2
overexpression can suppress several aspect of tumorigenesis. Further investigations are necessitated to verify if
NDRG2
molecule can be a therapeutic target in colorectal cancer.
...
PMID:Effects of NDRG2 Overexpression on Metastatic Behaviors of HCT116 Colorectal Cancer Cell Line. 2939 58
