Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.24.17 (
MMP-3
)
3,419
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Keratinocyte growth factor
(
KGF
) has been implicated in wound re-epithelialization and branching morphogenesis of several organs. To determine whether
KGF
induces these effects via induction of matrix metalloproteinase expression we have analysed the effect of
KGF
on the expression of
stromelysin
-2 in cultured HaCaT keratinocytes. Here we show a strong induction of
stromelysin
-2 mRNA within 5-8 h of stimulation of these cells with
KGF
. The degree of induction was similar to that achieved by treatment with epidermal growth factor or tumour necrosis factor alpha, whereas the stimulatory effect of transforming growth factor beta 1 was even stronger. To determine whether the induction of
stromelysin
-2 expression by growth factors and cytokines might be important for wound healing, we analysed the expression of this gene during the healing process of full-thickness excisional wounds in mice. Whereas
stromelysin
-2 mRNA could hardly be detected in unwounded skin, a biphasic induction was seen after injury and highest levels were found at days 1 and 5 after wounding. Hybridization in situ revealed the presence of
stromelysin
-2 mRNA in basal keratinocytes at the wound edge but not in the underlying mesenchymal tissue. During impaired wound healing as seen in glucocorticoid-treated mice,
stromelysin
-2 expression was significantly increased compared with untreated control mice. Taken together, these results suggest that correct regulation of this broad-spectrum metalloproteinase might be important for normal repair.
...
PMID:Regulation of the expression of stromelysin-2 by growth factors in keratinocytes: implications for normal and impaired wound healing. 897 81
