Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.23.5 (
cathepsin D
)
4,130
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The mode of degradation of myofibrillar proteins by the action of highly purified rabbit muscle
cathepsin D
(
EC 3.4.23.5
) was studied using SDS-polyacrylamide gel electrophoresis. Cathepsin D optimally degraded myosin heavy chain, alpha-actinin, tropomyosin,
troponin T
and troponin I at around pH 3. It did not degrade actin or troponin C. Degradation of myosin heavy chain produced four major fragments of 155000, 130000, 110000 and 90000 daltons. Troponin T was hydrolyzed to 33000-, and 20000- and 11000-dalton fragments. Troponin I was degraded into fragments of 13000 and 11000 daltons. Degradation of alpha-actinin and tropomyosin was not as rapid as that of myosin and troponins T and I. Tropomyosin gave a fragment of 30000 daltons, but alpha-actinin showed no distinct band of this fragment on gels.
...
PMID:Mode of degradation of myofibrillar proteins by rabbit muscle cathepsin D. 682 29
In this study, we examined the contribution of the cathepsins (
cathepsin D
and crude cathepsins containing cathepsins B and L) to
troponin T
degradation during postmortem aging. The action of
cathepsin D
on
troponin T
was examined at various pHs (pH 4.0-6.5). The degradation of intact
troponin T
was observed at pH 4.0, but not observed at pH 5.5 and 6.5. As a result of the degradation of
troponin T
, the 30 kDa fragment was not generated in any pH condition. The action of the crude cathepsins on
troponin T
was also examined at various pHs (pH 4.0-6.5). The intact
troponin T
was degraded at pH 4.0 and the 30 kDa fragments were observed. These 30 kDa fragments disappeared during further incubation. On the other hand, at pH 5.5 and 6.5, the intact
troponin T
was degraded and the 30 kDa fragment was accumulated. These results suggested that the
cathepsin D
scarcely contributed to the degradation of
troponin T
during postmortem aging, but crude cathepsins containing cathepsins B and L were partially involved in the degradation of
troponin T
and the generation of 30 kDa fragments.
...
PMID:Actions of cathepsins on troponin T during postmortem aging of porcine muscle. 2066 21
