Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.23.5 (
cathepsin D
)
4,130
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The pathogenesis of peptic ulcer is a complex phenomenon and several factors are thought to be involved in this process. Among others,
Helicobacter pylori infection
, hypergastrinaemia and some proteases seem to play an essential role in inducing peptic ulceration. We investigated whether tryptase (a serine endoprotease released by mast cells) and
cathepsin D
(a lysosomal hydrolase which seems able to derange the extracellular matrix) play a part in peptic ulcer disease and whether they are linked to
Helicobacter pylori infection
and mucosal content of gastrin. We studied 13 controls, 25 patients with gastric ulcer, 47 with duodenal ulcer and 11 with duodenitis. Tryptase and
cathepsin D
were measured in mucosal biopsy specimens (body and antrum of the stomach and duodenum) using IRMA methods. Gastrin was assayed in the antral mucosa by means of a RIA method.
Helicobacter pylori infection
was histologically evaluated (Giemsa). Tryptase and
cathepsin D
levels were higher (25%) in patients with active peptic ulcer, whether gastric or duodenal. The mucosal content of
cathepsin D
, but not that of tryptase, was associated with
Helicobacter pylori infection
. Tryptase, on the other hand, was related to gastrin content. No correlation was found between the two enzymes. It is concluded that tryptase and
cathepsin D
probably reflect different pathophysiological modifications in ulcer disease. Cathepsin D seems to be mainly related to the phlogistic reaction of the mucosa to
Helicobacter pylori infection
; tryptase may reflect and indirect link between the action of gastrin and the function of mast cells.
...
PMID:Are tryptase and cathepsin D related to Helicobacter pylori infection and mucosal gastrin in peptic ulcer? 820 35
The presence of
cathepsin D
was assessed in epithelial and stromal cells of the gastric mucosa in patients with ulcer disease depending on the course of ulcer healing, accompanying
Helicobacter pylori infection
, mode of treatment, location of ulceration, the presence of relapses, intestinal metaplasia, ulcer diameter, age and gender of the patients. The presence of
cathepsin D
was assessed by immunohistochemistry using an immunoenzymatic method StreptABC. Immunohistochemistry was performed on formalin-fixed paraffin sections obtained from 54 selected patients (20 men and 34 women, aged from 23 to 75 years), in whom endoscopy of the upper alimentary tract revealed ulcerations within the stomach, and then ulcer healing was monitored by endoscopy and histopathology. Before immunohistochemistry each specimen was routinely stained with hematoxylin and eosin, and histopathological study was performed to confirm a nonneoplastic character of ulcers, followed by staining with Giemsa method for the presence of Helicobacter pylori. The presence of
cathepsin D
was analyzed using image analysis. Student's t-test and a correlation coefficient were used to study the relationship between the expression of
cathepsin D
and selected parameters. The following significant relationships were observed: 1. between the expression of
cathepsin D
in epithelial cells and the course of peptic ulcer healing after antiulcerative treatment; 2. between the expression of
cathepsin D
in stromal cells after antiulcerative treatment and the course of ulcer healing.
...
PMID:Immunocytochemical evaluation of gastric mucosal cathepsin D in peptic ulcer. 979 10
