Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.4.23.5 (cathepsin D)
 4,130 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Rabbit antiserum against human liver cathepsin D was raised. The antiserum did not cross-react with cathepsins D from tissues of other species (bovine liver, bovine spleen, chicken liver). The study of cathepsins D isolated from human pathologically altered tissues showed that cathepsins from kidney malignant tumor and from myeloleucosis-induced spleen tumor were immunologically identical to the enzyme from normal liver. Cathepsins from liposarcoma and uterine myoma were characterized by partial identity with the enzyme from normal liver. Cathepsins D isolated from various human livers exhibited individual quantitative differences in antigenic properties, a fact to be taken into account in development of an immunochemical method for identification of cathepsin D. The low immunogenicity of human cathepsin D for rabbits and inadequate suitability of these animals for raising appropriate antisera was also considered.
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PMID:[Immunochemical studies of human cathepsin D]. 692 40

The lipid-rich residual bodies (LRRB) (Eyden et al., 1991) in human myometrium and uterine leiomyoma cells, have a distinctive ultrastructure characterised by a rich lipid content. To evaluate the biological or pathological significance in detail, normal myometrium and uterine leiomyoma from 30 human cases were studied by conventional histological, histochemical, immunohistochemical and electron microscopic methods. The study included a quantitative analysis of LRRBs of 3 premenarchic cases, 19 cases having a menstrual cycle, and 8 cases in menopause, in addition to 20 patients with histologically conventional leiomyoma larger than 3 cm in diameter. The study revealed the following findings: 1) immunohistochemical distribution of cathepsin D in the LRRB; 2) histochemical demonstration of neutral fat as the main content of LRRB; 3) statistically significant decrease in the distribution of LRRB in leiomyoma tissue compared with normal myometrium; 4) an absence or minimal distribution of LRRB in premenarchic myometrium; 5) a moderately significant correlation between the frequency of LRRB and patient's age. The distribution of cathepsin D within LRRB and the differential expression of LRRBs in the various smooth muscle cell tissues of the uterus suggest a possible role of ovarian hormones in the genesis of LRRBs which may function in the intra-lysosomal degradation of organelles produced during hormonal cycling.
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PMID:Lipid-rich residual bodies and cathepsin D in the human uterus: ultrastructural and quantitative comparison between normal myometrium and leiomyoma. 840 43