Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.23.5 (
cathepsin D
)
4,130
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
We previously demonstrated that the aspartate protease
cathepsin D
is activated by ceramide derived from acid sphingomyelinase. Increased expression of
cathepsin D
in the skin has been reported in wound healing, psoriasis and skin tumors. We explored specific functions of
cathepsin D
during epidermal differentiation. Protein expression and enzymatic activity of
cathepsin D
increased in differentiated keratinocytes in both stratified organotypic cultures and in mouse skin during epidermal barrier repair. Treatment of cultured keratinocytes with exogenous
cathepsin D
increased the activity of transglutaminase 1, known to cross-link the cornified envelope proteins involucrin and loricrin during epidermal differentiation. Inhibition of
cathepsin D
by pepstatin A suppressed the activity of transglutaminase 1. Cathepsin D-deficient mice revealed reduced transglutaminase 1 activity and reduced protein levels of the cornified envelope proteins involucrin and loricrin. Also, amount and distribution of cornified envelope proteins involucrin, loricrin, filaggrin, and of the keratins K1 and K5 were significantly altered in
cathepsin D
-deficient mice. Stratum corneum morphology in
cathepsin D
-deficient mice was impaired, with increased numbers of corneocyte layers and faint staining of the cornified envelope only, which is similar to the human
skin disease
lamellar ichthyosis. Our findings suggest a functional link between
cathepsin D
activation, transglutaminase 1 activity and protein expression of cornified envelope proteins during epidermal differentiation.
...
PMID:Cathepsin D is involved in the regulation of transglutaminase 1 and epidermal differentiation. 1512 30
