Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.23.16 (
HIV-1 protease
)
2,107
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
An inhibitor of the
HIV-1 protease
has been employed in the generation of a resin which allows the rapid purification of this enzyme. A peptide substrate analogue,
H2N
-Ser-Gln-Asn-(Phe-psi[CH2N]-Pro)-Ile-Val-Gln-OH, was coupled to agarose resin. The
HIV-1 protease
was expressed in E. coli and the supernatant from lysed cells was passed through the affinity resin. Active
HIV-1 protease
was then eluted with a buffer change to pH 10 and 2 M NaCl. Final purification to a homogeneous preparation, capable of crystallization, was achieved with hydrophobic interaction chromatography. Solutions containing
HIV-1 protease
bound to competitive inhibitors do not bind to the column.
...
PMID:Affinity purification of the HIV-1 protease. 268 42
