Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.23.16 (
HIV-1 protease
)
2,107
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Infection of T cells with HIV-1 induces apoptosis and modulates apoptosis regulatory molecules. Similar effects occur following treatment of cells with individual HIV-1 encoded proteins. While
HIV-1 protease
is known to be cytotoxic, little is known of its effect on apoptosis and apoptosis regulatory molecules. The ability of
HIV-1 protease
to kill cells, coupled with the degenerate substrate specificity of
HIV-1 protease
, suggests that
HIV-1 protease
may activate cellular factor(s) which, in turn, induce apoptosis. We demonstrate that
HIV-1 protease
directly cleaves and activates procaspase 8 in T cells which is associated with cleavage of BID, mitochondrial release of
cytochrome c
, activation of the downstream caspases 9 and 3, cleavage of DFF and PARP and, eventually, to nuclear condensation and DNA fragmentation that are characteristic of apoptosis. The effect of
HIV-1 protease
is not seen in T cell extracts which have undetectable levels of procaspase 8, indicating a specificity and requirement for procaspase 8.
...
PMID:HIV-1 protease processes procaspase 8 to cause mitochondrial release of cytochrome c, caspase cleavage and nuclear fragmentation. 1240 16
