Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.23.16 (
HIV-1 protease
)
2,107
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
In human immunodeficiency virus-1 (HIV-1), reverse transcriptase (RT) is encoded as a 66 kDa protein, p66, in the Gag-Pol polyprotein. This protein is proteolytically cleaved by
HIV-1 protease
(PR) to finally generate a mature RT that is a heterodimer, composed of a p66 subunit and a p66-derived 51 kDa subunit, p51. In our prior work, we demonstrated that tRNA
Lys3
binding to p66/p66 facilitates efficient cleavage of p66 to p51 by PR. However, tRNA
Lys3
is known to be recruited to the virus by forming a complex with
lysyl-tRNA synthetase
(
LysRS
). Herein, we tested whether
LysRS
can have an effect on RT maturation
in vitro
. Importantly, our data show no significant differences in RT maturation in the presence of
LysRS
. Furthermore, no apparent p66/66 interaction with
LysRS
was observed. Although PR cleaved
LysRS
, it did not immediately release tRNA
Lys3
from
LysRS
. Thus, we conclude that a free fraction of tRNA
Lys3
, which is in equilibrium with a
LysRS
-bound form, interacts with p66/p66 without any additional mechanism involving release of tRNA
Lys3
from
LysRS
. Given that only transient tRNA
Lys3
-p66/p66 interaction is needed for efficient RT maturation, a small amount of free tRNA may be sufficient for this process. These studies reveal molecular level insights into RT maturation and will be useful for the design of cellular/viral experiments to better understand the role of tRNA in HIV-1 replication.
...
PMID:Effect of Lysyl-tRNA Synthetase on the Maturation of HIV-1 Reverse Transcriptase. 3268 28
